The invention discloses an occult blood fingerprint appearing agent, an occult blood fingerprintappearing cotton piece and application oftheoccult blood fingerprint appearing cotton piece. A polyphenylene ethynylene type conjugated polymer with amino on the side chain protected by t-butyloxycarboryl is synthesized through an Sonogashira coupling reaction, theprotecting group is removed through trifluoroacetic acid, and an aminated polyphenylene ethynylene fluorescence conjugated polymer is obtained and is dissolved in a solvent, so that the occult blood fingerprint appearing agent is obtained.A non-woven fabric is adopted as a basematerial and is soaked through the fingerprint appearing agent, so that the occult blood fingerprint appearing cotton piece is obtained; the cotton piece coversan object loaded with an occult blood fingerprint; and after uncovering the cotton piece, bright fingerprint lines can be observed under ultraviolet light of 415nm. According to the technical scheme,the occult blood fingerprint appearing agent provided by the invention is simple and convenient to operate, short in developing time, good in developing effect, and suitable for occult blood fingerprint developing on different objects; and particularly, by adopting the appearing cotton piece, the appearing cotton piece is portable, reusable, capable of realizing industrial production, and suitablefor occult blood fingerprint appearing of objects in various dimensions on sites with different situations.