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Method for preparing macroalgae antioxidant peptide

A technology of antioxidant peptides and giant algae, which is applied in the field of fermentation, can solve problems such as difficulty in misappropriation, achieve excellent anti-oxidation, improve skin anti-oxidation ability, and scavenge free radicals

Active Publication Date: 2018-06-26
GUANGZHOU KENENG COSMETICS RES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has never been reported to ferment macroalgae to produce antioxidant peptides
Due to the different sources of raw materials and the differences in the molecular structure of the raw materials, it is suitable for other species, such as the fermentation method of peanuts and soybean meal, and it is difficult to transfer to other species.

Method used

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  • Method for preparing macroalgae antioxidant peptide
  • Method for preparing macroalgae antioxidant peptide
  • Method for preparing macroalgae antioxidant peptide

Examples

Experimental program
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Effect test

Embodiment 1-24

[0035] Example 1-24 Preparation method of giant algae antioxidant peptide

[0036] (1) Preparation of macroalgae aqueous solution: after picking macroalgae, clean it up, crush macroalgae into powder, add distilled water and mix evenly, the volume consumption of said distilled water is 20-30mL / g based on macroalgae powder mass; then ultrasonic dispersion For dissolution, the ultrasonic power is 250-350w, the ultrasonic frequency is 25-30kHz, the temperature is 20-30°C, and the time is 10-20min; use a filter membrane of 0.22μm to remove impurities; Add 1-6% NaOH solution to adjust the pH value to 6.5-7.5, sterilize in an autoclave at 121° C. for 10-20 minutes, and cool to 25° C. to obtain a macroalgae aqueous solution.

[0037] (2) Preparation of strain seed liquid: the concentration is 1×10 8 ~1×10 9 The liquid seed liquids of Bacillus subtilis, Bacillus licheniformis and Aspergillus oryzae of CFU / g are jointly inoculated into the culture medium, wherein, the seed liquid of B...

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Abstract

The invention belongs to the field of fermentation and relates to a method for preparing macroalgae antioxidant peptide by fermentation. In the method, a liquid fermentation process is adopted, macroalgae are processed and then fermented with a mixed strain of bacillus subtilis, bacillus licheniformis and aspergillus oryzae, and purified to obtain the macroalgae antioxidant peptide. The prepared antioxidant peptide can be applied to cosmetics to scavenge free radicals, improve skin antioxidant capacity, and achieve anti-aging effects.

Description

technical field [0001] The invention belongs to the field of fermentation and relates to a method for preparing macroalgae antioxidant peptides by fermentation. Background technique [0002] Macrocystis pyrifera (L.) Ag belongs to the genus Macrocystis pyrifera (L.) Macroalgae is one of the fastest-growing plants in the world. Under suitable conditions, each macroalgae can grow 30 to 60 centimeters in a day, and a macroalgae can grow to more than 50 meters in a year. Macroalgae are the longest family in the algae kingdom. Most macroalgae can grow to tens of meters, and the longest can even reach 200 to 300 meters, weighing up to 200 kilograms. The macroalgae resources in the ocean are abundant and have great potential for development and utilization. It is a very good renewable biological resource. [0003] 80% of macroalgae is water, and it also contains crude protein, crude fat, carbohydrates, alginate, potassium, iodine, amino acids and trace elements, so it can extract...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P21/00C12N1/20C12N1/14C12R1/125C12R1/10C12R1/69
CPCC12N1/14C12N1/20C12P21/00C12P39/00
Inventor 李传茂刘培黄建珍刘德海向琼彪张伟杰张楚标
Owner GUANGZHOU KENENG COSMETICS RES CO LTD
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