In-vitro generation method for direct adventitious shoots of clematis plants

A technology of clematis and clematis, which is applied in the field of direct adventitious bud generation of clematis plants in vitro, and can solve the problems of low frequency of adventitious bud occurrence, unsuitable for sexual reproduction, and limited application of garden cultivation.

Inactive Publication Date: 2018-06-29
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Clematis plants have problems such as less fruit, long germination time, and low germination rate, so they are not suitable for sexual reproduction, and their applicatio

Method used

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  • In-vitro generation method for direct adventitious shoots of clematis plants
  • In-vitro generation method for direct adventitious shoots of clematis plants
  • In-vitro generation method for direct adventitious shoots of clematis plants

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Experimental program
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Effect test

Embodiment 1

[0076] (1) Preparation of basal medium (Mulan basal medium, ML medium for short): prepare basal medium according to Table 1, pH 5.8, and sterilize at 121° C. for 15-25 minutes. Both induction and elongation of adventitious buds use this medium.

[0077] Table 1List of components of ML medium

[0078]

[0079]

[0080] (2) Materials: Clematis freshly extracted branches in early March (acquired from Shanghai Institute of Biological Sciences, Chinese Academy of Sciences), counting 2-3 twigs from the top down. When collecting materials on the spot, care should be taken not to select materials with a high degree of lignification.

[0081] (3) Wash the fresh branches with tap water, blot the surface water droplets, disinfect with 70% ethanol for 30-90 seconds on the ultra-clean workbench, soak in 0.1% mercuric chloride for 10-14 minutes, rinse with sterile water 5-8 times, dry the water droplets on the surface of the sterile material with sterile filter paper, remove the ste...

Embodiment 2

[0092] The steps of this embodiment are basically the same as in Embodiment 1, except that Clematis celery leaf is used.

[0093] The experimental procedure was repeated three times, and the average number of regenerated plants produced by each stem section with internodes was similar to the number of regenerated plants obtained by the clematis in Example 1 of the present invention, and was highly consistent with the parental phenotype, and each node The reproduction coefficient of segment, adventitious bud induction rate, rooting rate, transplanting survival rate all can reach the similar result of the clematis of the embodiment of the present invention 1.

[0094] Therefore, the Clematis celery leaf cultivated by the method of the present invention has very high adventitious bud induction rate, rooting rate and transplanting survival rate.

Embodiment 3

[0096] The steps of this embodiment are basically the same as those in Embodiment 1, except that Clematis broadleaf (also known as Clematis whole margin) is used.

[0097] The experimental procedure was repeated three times, and the average number of regenerated plants produced by each stem section with internodes was similar to the number of regenerated plants obtained by the clematis in Example 1 of the present invention, and was highly consistent with the parental phenotype, and each node The reproduction coefficient of segment, adventitious bud induction rate, rooting rate, transplanting survival rate all can reach the similar result of the clematis of the embodiment of the present invention 1.

[0098] Therefore, the broad-leaved clematis cultivated by the method of the present invention has very high adventitious bud induction rate, rooting rate and transplanting survival rate.

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Abstract

The invention provides an in-vitro generation method for direct adventitious shoots of clematis plants. Specifically, the invention provides a reproduction method for clematis plants. The method comprises the following steps: (a) performing sterile culture on stems of the clematis plants so as to obtain stems with axillary buds; (b) removing the axillary buds of the stems obtained in the step (a),performing induction culture, and growing adventitious buds; (c) culturing the adventitious buds obtained in the step (b), and rooting to obtain plants. According to the method disclosed by the invention, the stems germinated from the clematis plants in a sterile state are taken as initial explants for the first time, in-vitro reproduction is performed, formation of adventitious buds is directlyinduced without a callus formation stage, and after elongating, rooting and seeding forming after transplanting of regenerated bud seedlings, a large amount of clematis plants with high regenerated plant phenotype and excellent genetic stability can be obtained.

Description

technical field [0001] The invention relates to the field of cultivation of clematis plants, in particular to a method for direct adventitious bud generation of clematis plants in vitro. Background technique [0002] Clematis (Clematis) is a perennial woody or herbaceous vine, or an erect shrub or herb. There are about 300 species in the world, distributed in tropical and subtropical regions. There are about 108 species in my country, which are distributed all over the country. Clematis plants have both medicinal and ornamental functions. The plants contain triterpenoid saponins, alkaloids, coumarins, lignans and flavonoids, which have pharmacological effects such as antibacterial, anti-inflammatory, anti-tumor and analgesic. It has various flower shapes and colorful colors, and is known as the "Queen of Fujimoto". There are more than 600 cultivated varieties, which have been used in urban gardens and are highly respected by home gardening enthusiasts. Clematis can be dotte...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 朱木兰奉树成刘航
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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