Sputum specimen preparation staining method

Abstract

The invention relates to a sputum specimen preparation staining method which comprises the following steps: collecting a to-be-detected sputum specimen, mixing the sputum specimen and sputum treatmentliquid, and shaking to completely decompose the mixture; transferring the mixture into a test tube to perform oscillatory centrifugation after the sputum solution is completely decomposed, removing the supernatant, injecting cell preservation liquid into the test tube, and performing preparation by an automatic preparation staining machine, wherein the sputum treatment liquid comprises the following components in percentage by weight: 2-8% of phosphate, 2-3% of phenol, 2-3% of bromhexine, 20-40% of ethanol and 46-74% of distilled water. The sputum specimen preparation staining method providedby the invention is simple in process and small in usage amount of the sputum treatment liquid, and when the method is matched with the automatic preparation staining machine, qualified staining sections can be prepared on a large scale at a time and are stable in quality, and the single speed can reach 2 sections per minute.

Description

technical field [0001] The invention belongs to the technical field of cell slice preparation and staining, and in particular relates to a method for preparing and staining sputum specimen slices. Background technique [0002] Sputum is a secretion produced by the trachea, bronchi, and alveoli. Under normal circumstances, this kind of secretion is very little. Under pathological conditions such as bacterial infection, such as lung inflammation, tuberculosis, and tumors, the amount of sputum increases, and its properties and components change. Sputum specimens are the most common specimens for clinical microbiology testing. Collecting sputum specimens, making slides and staining them for observation and analysis is of great significance for the diagnosis of diseases. [0003] Sputum pathogen detection methods include sputum smear method and sputum culture method. The former method is widely used all over the world. It has the advantages of fast, simple and cheap, but the pos...

AI Technical Summary

Problems solved by technology

[0003] Sputum pathogen detection methods include sputum smear method and sputum culture method. The former method is widely used all over the world. It has the advantages of fast, simple and cheap, but the positive rate is low and it is easily affected by sample preparation equipment and reagents; The latter has higher sensitivity, specificity, accuracy, and reliable results, but the culture period is longer and the efficiency is not as good as that of the smear method.