Antineoplastic application of apios americana medikus flower polysaccharide
An anti-tumor and taro flower technology, applied in the field of medicine, can solve the problems of few reports on anti-tumor activity, achieve significant anti-tumor activity, expand application fields, and have good development prospects
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Embodiment 1
[0041] Embodiment 1, the antitumor application of taro flower polysaccharide, such as Figure 1-Figure 8 As shown, taro flower polysaccharide has significant anti-tumor effect.
[0042] The specific preparation steps of taro flower polysaccharide are as follows:
[0043] (1) Weigh 100g of taro flowers, add 500mL of distilled water according to the ratio of material to liquid: 1g: 5ml, inactivate the enzyme in a water bath at 90°C for 80min, then beat the pulp, centrifuge at 4000r / min for 15min, take the supernatant and collect the filtrate .
[0044] (2), replace the taro flower in step (1) with the filter residue obtained by centrifugation in step (1), repeat the extraction 2 times, combine the filtrate three times, and concentrate by rotary evaporation to obtain a concentrated solution (concentrated by rotary evaporation to 1 / 5 of the original volume) .
[0045] (3) Add pure ethanol to the concentrated solution obtained in step (2) under agitation until the final concentr...
Embodiment 2
[0055] Embodiment 2, the effect of taro flower polysaccharide on the tumor volume of mice;
[0056] The mouse tumor volume (before putting to death) after intragastric gavage in the measurement embodiment 1 28 days, the result is as follows figure 2 As shown, the tumor volume in the control group was the largest. Compared with the control group, the positive control group and the intervention group with different concentrations of taro flower polysaccharides had a certain inhibitory effect on the increase of tumor volume, and the tumor volume in the positive drug group was the smallest. The above results showed that taro flower polysaccharide can alleviate the increase of tumor volume in tumor-bearing nude mice.
Embodiment 3
[0057] Embodiment 3, the influence of taro flower polysaccharide on mouse tumor weight and tumor inhibition rate;
[0058] After 28 days of intragastric administration in Example 1, the mice were fasted for 12 hours, and the mice were killed by cervical dislocation after blood was collected from the orbits. According to the formula: tumor inhibition rate=(1-weight of tumor mass in experimental group / weight of tumor mass in control group)*100%. The result is as image 3 , Figure 4 As shown, both the positive control group and the intervention group of taro flower polysaccharides with different concentrations had inhibitory effects on tumor growth, and the positive drug group had the highest tumor inhibition rate.
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