Antitumor application of apios americana flower ethanol extract

An ethanol extraction and anti-tumor technology, applied in the field of medicine, can solve the problem of rarely reported anti-tumor activity, and achieve the effects of significant anti-tumor activity, expanding application fields and good development prospects.

Inactive Publication Date: 2018-08-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, at present, many nutrition and health food researchers are very concerned about the research and topic of tumor prevention, but there are few reports on the antitumor activity of taro flower extract

Method used

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  • Antitumor application of apios americana flower ethanol extract
  • Antitumor application of apios americana flower ethanol extract
  • Antitumor application of apios americana flower ethanol extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, the anti-tumor application of the ethanol extract of beetroot flower, such as Figure 1-Figure 8 As shown, the ethanol extract of taro flower has significant antitumor effect.

[0048] The specific preparation steps of the taro flower ethanol extract are as follows:

[0049] (1) According to the solid-liquid ratio of 1g: 10ml, after mixing the taro flower and the ethanol containing 0.1mol / L hydrochloric acid, it is processed and beaten by a juice extractor. The obtained slurry was ultrasonically leached for 90 minutes at 45° C., the extract was centrifuged at 3000 g for 30 minutes, and the extract was centrifuged to obtain a supernatant.

[0050] (2), replace the taro flower in step (1) with the centrifuged filter residue obtained in step (1), repeat the extraction 2 times, combine the filtrate three times, and concentrate by rotary evaporation to obtain a concentrated solution (concentrated by rotary evaporation to 15% of the original volume).

[0051] ...

Embodiment 2

[0062] Embodiment 2, the influence of the ethanol extract of japonica flower on the tumor volume of mice;

[0063] The mouse tumor volume (before putting to death) after intragastric gavage in the measurement embodiment 1 28 days, the result is as follows figure 2 As shown, the tumor volume in the control group was the largest. Compared with the control group, the positive control group and the intervention group with ethanol extracts of different concentrations of bean taro flowers had a certain inhibitory effect on the increase of tumor volume, and the tumor volume in the positive drug group was the smallest. The above results show that the ethanol extract of taro flower can alleviate the increase of tumor volume in tumor-bearing nude mice.

Embodiment 3

[0064] Embodiment 3, the influence of ethanol extract of taro flower on mouse tumor weight and tumor inhibition rate;

[0065] After 28 days of intragastric administration in Example 1, the mice were fasted for 12 hours, and the mice were killed by cervical dislocation after blood was collected from the orbits. According to the formula: tumor inhibition rate=(1-weight of tumor mass in experimental group / weight of tumor mass in control group)*100%. The result is as image 3 , Figure 4 As shown, the positive control group and the intervention groups of ethanol extracts of taro flower with different concentrations all had inhibitory effects on tumor growth, and the positive drug group had the highest tumor inhibition rate.

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Abstract

The invention discloses antitumor application of an apios americana flower ethanol extract. The antitumor application is characterized in that subcutaneous area of a nude mouse is inoculated with a hepatoma carcinoma cell Huh7 to form a tumor; meanwhile, the intervention of the apios americana flower ethanol extract is performed, and change conditions of body weight of the nude mouse in the experiment process are monitored. After an intervention experiment is ended, a subcutaneous tumor is stripped off, the weight of the tumor is recorded and secretion conditions of cytokines in serum are monitored, so that the intervention of the apios americana flower ethanol extract has obvious antitumor effect.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an antitumor application of an ethanol extract of bean taro flower. Background technique [0002] Liver disease, in modern medicine, refers to diseases of the liver organ based on anatomical morphology, such as viral acute, chronic hepatitis, cirrhosis, schistosomal liver disease, fatty liver, liver cysts, and hepatic cavernous hemangiomas. The main cause of liver disease is hepatitis B virus infection, and the excessive compensatory proliferation of liver cells caused by long-term chronic inflammation can induce liver cancer. About 2 billion people in the world have been infected with hepatitis B, and 30% of the 350 million chronic hepatitis B virus carriers develop into liver cirrhosis; the number of people infected with hepatitis B virus in my country reaches 120 million, and about 20% of patients with chronic hepatitis develop into liver cirrhosis and liver cancer. %-90% of liver canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P35/00A23L33/105A61K133/00
CPCA61K36/48A23L33/105A23V2002/00A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61P35/00A23V2250/21A23V2200/308
Inventor 郑晓冬楚强陈鹏华刘阳阳李永璐俞露霜
Owner ZHEJIANG UNIV
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