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High-efficiency and high-sensitivity biomacromolecular membrane transferring and dyeing system and equipment

A biomacromolecule, high-sensitivity technology, applied in the field of biological detection equipment, can solve the problems of slow movement and easy detection of missed detection, achieve smooth and even close contact, reduce environmental pollution and harm to operators, and ensure sensitivity Effect

Inactive Publication Date: 2018-08-10
NANJING GENSCRIPT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the factors affecting the electrophoretic mobility are mainly related to the electric field strength, the pH value of the solution, and the ionic strength of the solution, the mobility of biomacromolecules and dyes in the medium where the electric field is applied is not the same, and biomacromolecules are larger. It moves slowly in the electric field and will not move out of the medium within a limited time. Organic dye molecules are much smaller than biomacromolecules, and dye molecules that are not combined with biomacromolecules can quickly move out of the medium, so they can be In the same electric field, the color development of biomacromolecules can be achieved by moving the dye out of the medium while the biomacromolecule (incorporated with the dye) remains in the medium, so that high resolution, little or even no background influence can be obtained in a short time. Analytical images of biomacromolecules in the background, but if the dye and biomacromolecules only meet and combine once, it is difficult to meet the requirements for some experiments with relatively high sensitivity requirements, and even prone to missed detection in the detection
[0005] With the rapid development of biomedicine and other disciplines, the detection technology of biomacromolecules is also rapidly improving. However, there is no system or device in the market that can use electric fields to drive the movement of biologically charged particles to control the rapid dyeing and transfection of biomacromolecules. membrane, and at the same time achieve the requirement of high sensitivity

Method used

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  • High-efficiency and high-sensitivity biomacromolecular membrane transferring and dyeing system and equipment
  • High-efficiency and high-sensitivity biomacromolecular membrane transferring and dyeing system and equipment
  • High-efficiency and high-sensitivity biomacromolecular membrane transferring and dyeing system and equipment

Examples

Experimental program
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Effect test

Embodiment 1

[0051] A high-efficiency and high-sensitivity biomacromolecule transmembrane and staining system that can achieve different sensitivities for different detection purposes, including a working unit with 2-6 channels for detecting and analyzing samples; and associated with the working unit In the operation part, 2-6 dyeing or membrane transfer channel parameters are correspondingly created, and each dyeing or membrane transfer channel parameter is set to a different number of electric field cycles according to different detection purposes and required sensitivities.

Embodiment 2

[0053] Such as figure 1 A system for accelerating the detection of biomacromolecules is shown, which includes a working unit, which is provided with 4 channels for detecting and analyzing samples; an operating part associated with the working unit, which can simultaneously create 4 staining or transmembrane Channel parameters, such as: channel label, dyeing / transfer, Gel number, working time, working voltage, working current, electric field cycle times, information confirmation, start / pause, analysis / processing of input data, etc.; monitoring equipment for monitoring In the normal operation of the operation, when a specific channel in the working unit of the machine does not put the sample to be analyzed, or puts it in wrongly, the machine does not work and prompts, and it can also judge whether the work of each channel is completed, mainly including: machine work Work according to the set time, end the work after the time is up and prompt the end of the work or the machine ca...

Embodiment 3

[0067] A device based on a high-efficiency and high-sensitivity biomacromolecule transfer film and dyeing system, including a base and a housing for providing internal and external support for the machine. There are touch buttons on the top of the housing, and there are The working area and the system power supply and working circuit board connected to the working area; 2-6 pull-out independent studios are set in the working area, and the positive and negative electrode plates that provide the electric field are installed in the working area.

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Abstract

The invention discloses a high-efficiency and high-sensitivity biomacromolecular membrane transferring and dyeing system, which comprises a work unit and an operation part, wherein the work unit is provided with at least one passage for detecting analysis samples; the operation part is associated with the work unit; the operation part creates at least one dyeing or membrane transferring passage parameter; each dyeing or membrane transferring passage parameter comprises at least one electric field circulation time. The invention also discloses equipment based on the high-efficiency and high-sensitivity biomacromolecular membrane transferring and dyeing system. The equipment comprises a base and a casing, wherein an operation panel is arranged outside the casing; a work region, a system power supply and a work circuit board are arranged in the base and the casing; the system power supply and the work circuit board are connected with the work region; the work region is at least provided with a pulling type independent work chamber; a positive and negative electrode board used for providing an electric field is arranged in the work chamber. The system or equipment capable of controlling the fast biomacromolecular membrane transferring and dyeing through the motion of bioelectric particles driven by the electric field is provided; meanwhile, the high-sensitivity requirement of the biological detection can also be ensured.

Description

[0001] This application is a divisional application with application number 201310025508.2, application date 2013.01.22, and application name "An Efficient and Highly Sensitive Biomacromolecule Transfer and Staining System and Equipment". technical field [0002] The invention relates to the technical field of biological detection equipment, in particular to a biomacromolecule transmembrane and dyeing system and equipment that utilizes an electric field to drive the movement of organisms or other charged particles to achieve high efficiency and high sensitivity. Background technique [0003] In a DC electric field, the phenomenon that charged particles move to electrodes with opposite signs is called electrophoresis (electrophoresis). In 1809, the Russian physicist Peнce first discovered the phenomenon of electrophoresis, but it was not until 1937 that Tiselius in Sweden established the boundary electrophoresis (boundary electrophoresis) for separating proteins that the elect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/31
Inventor 不公告发明人
Owner NANJING GENSCRIPT BIOTECH CO LTD
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