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Compounds extracted from Euphorbia neriifolia to treat cancers

A compound and squamous cell carcinoma technology, applied in organic chemistry, drug combination, antineoplastic drugs, etc., can solve the problems of unclear active components and application limitations, and achieve the effect of definite pharmacological mechanism of action

Inactive Publication Date: 2018-08-17
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, in view of the problems of unclear active components and limited application of diamond powder in the prior art, the present invention provides two active substances separated from diamond powder, which have anticancer activity and good activity effect

Method used

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  • Compounds extracted from Euphorbia neriifolia to treat cancers
  • Compounds extracted from Euphorbia neriifolia to treat cancers
  • Compounds extracted from Euphorbia neriifolia to treat cancers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Compound 1 and Compound 2 on the proliferation of normal cells and esophageal squamous cell carcinoma cells

[0039] 1. Experimental materials, instruments and reagents

[0040] 1 Experimental materials

[0041] 1.1 Drugs: compound 1 and compound 2 separated by our laboratory

[0042] Docetaxel (Jiangsu Hengrui Medicine Co., Ltd.)

[0043] 1.2 Cell lines:

[0044] Human normal esophageal epithelial cells (HEEC) and human esophageal squamous cell carcinoma cell lines (TE-13, KYSE-450, KYSE-410) are preserved by our research group.

[0045] 2 Preparation methods of main test reagents

[0046] 2.1 RPMI-1640 preparation method: take 10.4g RPMI-1640 dry powder, 2g NaHCO 3 , 50mg penicillin, 100mg streptomycin, ddH 2 O 800mL, fully mixed with a magnetic stirrer, adjusted to pH 7.4 with HCl, sterilized by filtration with a 0.22μm filter, aliquoted, and stored at 4°C for later use.

[0047] 2.2 PBS liquid preparation method: NaCl 8.0g, KCl 0.2g, NaCl 2 HPO 4 ...

Embodiment 2

[0070] Example 2 Effect of compound 1 and compound 2 on the proliferation of liver cancer cells

[0071] 2. Experimental materials, instruments and reagents

[0072] 1 Experimental materials

[0073] 1.1 Drugs: compound 1 and compound 2 separated by our laboratory

[0074] 1.2 Cell line: HepG-2 cells, preserved by our research group.

[0075] 2 Preparation methods of main test reagents

[0076] 2.1 DMEM medium preparation method: take DMEM dry powder, 2g NaHCO 3 , 50mg penicillin, 100mg streptomycin, ddH 2 O 800mL, fully mixed with a magnetic stirrer, adjusted to pH 7.0 with HCl, adjusted to 1000ml with double distilled water, sterilized with a 0.22μm filter, aliquoted, and stored at 4°C for later use.

[0077] 2.2 PBS liquid preparation method: NaCl 8.0g, KCl 0.2g, NaCl 2 HPO 4 1.44g, KH 2 PO 4 0.24g, add water to make up to 1L, sterilize under high temperature and high pressure, and store at 4°C for later use.

[0078] 2.3 Preparation method of trypsin: dissolve in...

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Abstract

The invention belongs to the technical field of medicine and particularly relates to two compounds extracted from Euphorbia neriifolia. The compounds may function as active compounds to treat cancers.The invention also relates to a pharmaceutical composition using the compounds as effective ingredients and application of thereof in the preparation of anticancer drugs. The compounds have the one of the following structures shown in the description. The compounds are extracted from Euphorbia neriifolia by isolating; MTT (methyl thiazolyl tetrazolium) process is used to perform activity detection on the compounds isolated from Euphorbia neriifolia; the antitumor activity of the compounds is proved.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to two compounds extracted from Japonica, which can be used as active compounds for treating cancer, a pharmaceutical composition with the same as an active ingredient, and its application in the preparation of anticancer drugs. Background technique [0002] Malignant tumor is one of the common diseases that seriously endanger human life and health worldwide. Its harm has surpassed cardiovascular and cerebrovascular diseases and has become the first cause of death for human beings. The World Health Organization has called it the number one killer of human life. [0003] Tumor cells usually have three basic characteristics, namely, uncontrolled proliferation, insufficient differentiation and blocked apoptosis. At present, apart from surgery, radiotherapy and chemotherapy are still the main treatment methods for tumors, but they are mainly designed for the uncontrolled p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/21A61P35/00
CPCA61P35/00C07C69/21
Inventor 戚微岩董瑶徐寒梅
Owner CHINA PHARM UNIV
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