The invention relates to the technical field of facial beautification, and discloses a marine collagen firming sleeping mask and a preparation method thereof. The marine collagen firming sleeping mask comprises deionized water, a humectant, a thickening agent, a solubilizer, marine collagen, a rice fermentation product filtrate, a tranquilizing plant extract, panthenol, aloe pulp, honey and lemon essence; according to the mask, the marine collagen, the rice enzyme fermentation filtrate and various natural plants and medicinal components are utilized, so that the effects of nourishing skin, improving facial wrinkles, repairing damaged skin, moisturizing skin, purifying deep dirt, relieving facial sensitivity and the like are achieved, water retention, tightening and pore shrinking are effectively performed on human skin, a mask cloth medium does not need to be used. The rayon garment skin is prevented from running to the membrane cloth, the membrane cloth is not needed, and environmental protection is facilitated; by means of various nerve soothing plant extracting solutions, the medicinal effect of the mask is further enhanced, nerve soothing and sleep aiding are conducted on the human body when people use the mask, and the sleep quality of a user is improved.