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Method for using liquid chromatogram-tandem mass spectrometry to measure concentration of glipizide in plasma

A liquid chromatography and glipizide technology, applied in the field of determination of glipizide concentration in beagle dog plasma, can solve the problem of high detection limit

Inactive Publication Date: 2018-08-31
沈阳信达泰康医药科技有限公司
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Problems solved by technology

It overcomes the problems of other methods such as complexity and high detection limit, and quickly detects the content of glipizide in beagle dog plasma, with sensitive, fast and reproducible results

Method used

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  • Method for using liquid chromatogram-tandem mass spectrometry to measure concentration of glipizide in plasma
  • Method for using liquid chromatogram-tandem mass spectrometry to measure concentration of glipizide in plasma
  • Method for using liquid chromatogram-tandem mass spectrometry to measure concentration of glipizide in plasma

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Embodiment Construction

[0075] The invention adopts the pretreatment technology of protein precipitation, and uses liquid chromatography tandem mass spectrometry to measure the content of glipizide in beagle dog plasma.

[0076] The concrete implementation method of the present invention is as follows:

[0077] (1) Preparation of stock solution

[0078] Accurately weigh two appropriate amounts of glipizide, dissolve in a volumetric flask with 10% methanol solution, constant volume, mix well, and use it as a stock solution for preparing a standard curve and a stock solution for preparing a quality control sample. Accurately weigh an appropriate amount of gliclazide, dissolve it in a volumetric flask with 10% methanol solution, constant volume, mix well as the stock solution of the internal standard solution, and store it in an 8-degree refrigerator.

[0079] (2) Preparation of working solution

[0080] Dilute the above glipizide stock solution with 10% methanol to prepare standard curve sample worki...

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Abstract

The invention belongs to the field of medicine detection, and relates to a method for using liquid chromatogram-tandem mass spectrometry to measure concentration of glipizide in plasma, in particularto a method for measuring the concentration of glipizide in plasma of a beagle. The method comprises the following steps of 1, preparation of a plasma sample; 2, sedimentation of protein; 3, measuringthrough the liquid chromatogram-tandem mass spectrometry. An interior label working solution gliclazide is added in the sample, and methyl alcohol is adopted for settling, volution and centrifuging.Then the liquid chromatogram-tandem mass spectrometry is adopted for measuring the concentration of glipizide in the plasma. The method adopts methyl alcohol for settling the protein, has the advantages that liquid chromatogram-tandem mass spectrometry is conducted, the operation is simple, the detection limit is low, and the repeatability is high, and is applicable to pharmacokinetic studies, toxicokinetic studies, bioequivalence studies and the like.

Description

technical field [0001] The method belongs to the field of medical detection, and relates to a method for determining the concentration of glipizide in plasma by liquid chromatography-tandem mass spectrometry, in particular to a method for determining the concentration of glipizide in beagle dog plasma. Background technique [0002] Due to the improvement of living standards and the aggravation of aging, the number of diabetic patients is on the rise year by year; according to incomplete statistics, the incidence of diabetes in all countries in the world is on the rise, and type II diabetes patients account for more than 90%. In recent years, the rising rate of diabetes in developed countries is 45%, and the rising rate in developing countries is as high as 200%. More and more data show that diabetes is very common in China, India, and some developing countries in Africa. common and popular. In recent years, the increase trend of diabetes patients in my country has been on t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 赵龙山秦峰刘俊丽佟羚
Owner 沈阳信达泰康医药科技有限公司
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