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A kind of polymer potassium ion fluorescent probe and its preparation method and application

A technology of fluorescent probes and potassium ions, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, and luminescent materials. And biocompatibility enhancement, high selectivity effect

Active Publication Date: 2021-07-30
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the water solubility of this small molecule probe is poor, and the detection limit range of this type of probe needs to be further improved

Method used

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  • A kind of polymer potassium ion fluorescent probe and its preparation method and application
  • A kind of polymer potassium ion fluorescent probe and its preparation method and application
  • A kind of polymer potassium ion fluorescent probe and its preparation method and application

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Embodiment 1

[0074] This embodiment provides a method of preparation of a high molecular potassium ion fluorescence probe P1, as follows:

[0075] (1) Synthetic polymer OEG-1

[0076] Polyethylene glycol monomethyl methacrylate (MPEG350-MMA, 1G), methacrylic acid (MAA, 60 mg), methacryloxyethyltrimethyl ammonium chloride (Metac, 50mg), Porphoine monom (2 mg) and azo diisobutyronitrile (AIBN, 20 mg) were added to 10 ml of Schlenlenk, and 5 ml of DMF was added. The mixed liquid is frozen with liquid nitrogen into solid state. After repeating the vacuum, thawing three times, nitrogen gas, closed valve, 65 ° C for 16 h. After the reaction was completed, the reaction solution was slowly added to methanol under ice bath conditions to give a white precipitate. The filtration was filtered and the methanol was rinsed 3, and the white solid OEG-1 (959.0 mg) was obtained, and the yield was 88.0%.

[0077] Structure Characterization: 1 H NMR (400MHz, D 2 O) δ: 4.10 (S, 2H), 3.63 (D, J = 64.5 Hz, 40H), 3.3...

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Abstract

The present invention relates to a polymeric potassium ion fluorescent probe and its preparation method and application. The probe is a ratiometric polymeric potassium ion fluorescent probe based on a water-soluble polymer framework and has a structure shown in P1. The present invention adopts The fully water-soluble polymer OEG-1 was obtained by living radical polymerization, and then the potassium ion sensor KS-23 was grafted through the esterification reaction between the carboxyl group in the polymer backbone and the hydroxyl group in the small molecule potassium ion probe KS-23 On the water-soluble polymer backbone, the ratiometric potassium ion fluorescent probe P1 based on the water-soluble polymer backbone is obtained. Compared with the small molecule potassium ion KS-23, the water solubility and biocompatibility of P1 are improved, and the potassium ion The selectivity is high, and the detection range of potassium ions is between 1-200mM, and it is expected to perform quantitative analysis on the concentration of potassium ions through peak ratio, which meets the requirements of quantitative detection of intracellular potassium ions.

Description

Technical field [0001] The present invention relates to the field of biomaterials, and more particularly to a high molecular potassium ion fluorescence probe and a preparation method thereof. Background technique [0002] Potassium ions (K + ) Is one of the most abundant metal ions in mammalian cells, accounting for about 4% of the human body. It plays and its important role in many physiological activities, including heart beat, muscle contraction, neural signal conduction and kidney excretion. K + Not only participate in maintaining intracellular osmotic pressure and electrolyte balance, regulating blood pressure, but also participated in the adjustment of the transduction of the biochemical signal of the entire nervous system. Mammalian cytoplasmic + The concentration of ions was about 130-180 mm, while the concentration of extracellular was 3.5-5.0 mm. The potassium ion level in normal serum is maintained between 3.5.5.5mm. When the excess is intake, it is usually removed by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/28C08F220/06C08F220/34C08F8/42C09K11/06G01N21/64
CPCC08F8/42C08F220/28C09K11/06C09K2211/14G01N21/6428
Inventor 田颜清宁爵伟
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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