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Duck infectious serositis living vaccine and preparation method thereof

A live vaccine and infectious technology, which is applied to the field of live attenuated vaccine against Riemerella anatipestifer and its preparation, can solve the problems such as no reports on the research of live vaccine of duck infectious serositis, and achieves good safety, Improves resistance and safety

Active Publication Date: 2018-09-04
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there is no domestic report on research on duck infectious serositis live vaccine

Method used

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  • Duck infectious serositis living vaccine and preparation method thereof
  • Duck infectious serositis living vaccine and preparation method thereof
  • Duck infectious serositis living vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Riemerella anatipestifer GD12 strain

[0031] 1. The source and preservation of the strain

[0032] The strain used for the production of the vaccine is Flavobacteriaceae, a new genus of Riemerella, and Riemerella anatipestifer GD12 strain, which was isolated by the inventor from the heart of ducklings in a duck farm in Guangdong Province in 2012, and was obtained in 2016. On April 1, it was sent to the China Center for Type Culture Collection for preservation, with the preservation number: CCTCC NO: M 2016161. It is identified by Gram staining, PCR technology, animal regression test, biochemical test and other methods.

[0033] 2. Strain characteristics

[0034] (1) Morphology of Riemerella anatipestifer GD12 strain

[0035] Methods: The duck heart was collected aseptically, streaked and inoculated on TSA plate and MacConkey agar plate, cultured in a candle jar at 37°C for 18-48 hours, and a single suspicious colony was picked for pure culture.

[0036] R...

Embodiment 2

[0074] Embodiment 2 Riemerella anatipestifer attenuated live vaccine (i.e. duck infectious serositis live vaccine) and preparation method thereof

[0075] Experimental material: Riemerella anatipestifer GD12 strain, isolated and identified from Guangdong Province in 2012 by the Parasite Laboratory of Animal Health Institute, Guangdong Academy of Agricultural Sciences, and sent to the China Type Culture Collection on April 1, 2016 Preserved by the central government, the deposit number is CCTCC M 2016161, and the test result on April 8, 2016 was survival. SPF ducks were purchased from the Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences for safety and immune efficacy tests.

[0076] The preparation steps of Riemerella anatipestifer attenuated live vaccine (i.e. duck infectious serositis live vaccine) are as follows:

[0077] 1) Strains

[0078] Inoculate the GD12 strain of Riemerella anatipestifer on a TSA (containing 5% newborn bovine seru...

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Abstract

The invention discloses a duck infectious serositis living vaccine and a preparation method thereof. The duck infectious serositis living vaccine disclosed by the invention contains a riemerella anatipestifer strain GD12 and a freeze-drying protective additive, wherein the riemerella anatipestifer strain GD12 is delivered to China Center for Type Culture Collection to be collected on April 1, 2016, and has a collection number of CCTCC NO: M 2016161. The duck infectious serositis living vaccine disclosed by the invention is high in safety and does not cause any adverse reactions to ducks; the living vaccine has an excellent immune effect, is capable of obviously improving resistance of ducklings to duck infectious serositis caused by type 1 riemerella anatipestifer, can be used for clinically preventing the duck infectious serositis caused by type 1 riemerella anatipestifer, and has significant economic and social benefits.

Description

technical field [0001] The invention belongs to the field of animal vaccines, and relates to an attenuated live vaccine of Riemerella anatipestifer (ie live duck infectious serositis vaccine) and a preparation method thereof. Background technique [0002] Riemerella anatipestifer, also known as duck infectious serositis, new duck disease, and duck plague syndrome, is a contact acute or chronic septicemia caused by Riemerella anatipestifer (RA). Infectious diseases, mainly affecting ducklings aged 2 to 7 weeks, clinically manifested as increased eye and nasal secretions, panting, coughing, diarrhea, ataxia and head and neck tremors, and chronic diseased ducks mainly manifested as meningitis Symptoms, neck tilt, slow growth or stiff ducks, lesions are mainly characterized by fibrinous pericarditis, perihepatitis and air sacculitis, and the mortality rate is as high as 90%, causing huge economic losses to the duck industry. The survey shows that duck infectious serositis is ub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
CPCA61K39/0208A61K2039/522A61K2039/552A61P31/04C12N1/20C12N1/205C12R2001/01
Inventor 吴彩艳董嘉文吕敏娜廖申权李林林孙敏华张建峰孙铭飞徐志宏
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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