29 micro-haplotype sites, screening method, multiplex amplification system and application

Abstract

The invention discloses 29 micro-haplotype sites, a screening method, a multiplex amplification system and an application. The screening method comprises the following steps: acquiring population data, acquiring micro-haplotype genotypes and allele frequencies from the population data, calculating forensic related parameters of candidate sites in different populations and excluding sites affectingsubsequent sequencing typing to obtain the 29 micro-haplotype sites; designing primers and amplification conditions according to the 29 micro-haplotype sites, and establishing the multiplex amplification system based on three-round PCR amplification systems. According to the multiplex amplification system, DNA templates extracted from mixed biological samples are amplified, amplification resultsare subjected to large-scale parallel sequencing, value of the sequencing depth of each micro-haplotype site in the DNA templates is obtained, and finally, the purpose of typing the mixed biological samples is achieved. The multiplex amplification system is suitable for large-scale parallel sequencing technology and has the advantages of high detection flux, high detection speed, large quantity ofobtained data, more accurate detection results and the like.

Description

technical field [0001] The invention belongs to forensic material evidence identification, and in particular relates to 29 micro-haplotype sites, a screening method, a compound amplification system and applications. Background technique [0002] Mixed biological samples refer to samples that are mixed with two or more biological components. Mixed biological specimens are a common type of difficult specimens in actual forensic prosecution. With the continuous advancement of scientific and technological means, more and more criminal cases have added the inspection and use of DNA in mixed biological samples. Mixed biological samples may appear in various types of cases, and most of them are sexual assault and violent injury (such as homicide, dismemberment) cases. Suspicious dander, skin swabs, or bodily fluid evidence collected by investigators at the scene often come from different individuals. Therefore, the DNA testing and determination of mixed biological samples plays ...

AI Technical Summary

Problems solved by technology

However, when the proportion of DNA from different individuals in the mixed biological specimens differs too much (less than 5% to 10%), due to the influence of factors such as dominant amplification, shadow peak (stutter), and random effects, the DNA components are less Few individuals can be successfully detected and typed

When Y-STR genetic markers are used, the detection ratio of male DNA components against female DNA background can be increased, but female components cannot be detected, and it is difficult to distinguish individuals with paternal genetic relationships, even with rapid mutations with high mutation rates (rapid mutating, RM) Y-STR genetic markers, about 50% of father-son pairs and 40% of sibling pairs cannot be distinguished

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