Application of aspidopterys obcordata hemsl and extract of aspidopterys obcordata hemsl to anti-tumor field

A technology of Radix vulgaris and saponins of Radix Radix are applied in the application field of Radix Radix and its extracts in anti-tumor, which can solve the problems such as anti-tumor effects of Radix Radix et al.

Active Publication Date: 2018-09-18
中国医学科学院药用植物研究所云南分所
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of aspidopterys obcordata hemsl and extract of aspidopterys obcordata hemsl to anti-tumor field
  • Application of aspidopterys obcordata hemsl and extract of aspidopterys obcordata hemsl to anti-tumor field
  • Application of aspidopterys obcordata hemsl and extract of aspidopterys obcordata hemsl to anti-tumor field

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Experimental program
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Effect test

Embodiment 1

[0032] (1) Get the dry cane of the scutellaria saponifera, cleanly select, cut into sections, extract with 85% ethanol, and the extract obtained after concentrating and drying the extract is subjected to D101-b macroporous resin chromatography to obtain 50% ethanol-water is used as the eluent for elution to obtain an extract, and the content of total saponins in the extract is 50.8%. Separation of total saponins from S. saponifera by silica gel column chromatography, gradient elution with 7% chloroform-methanol to obtain a refined extract, purifying the refined extract of S. The content of saponin A in the refined extract is 85.9%.

[0033] (2) Inhibitory effect of total saponins of Scutellaria saponica on H22 tumor-bearing mice

[0034] After H22 was passed three times in the peritoneal cavity of the mouse, the ascites was taken and diluted with normal saline to obtain 1×10 7 / mL -1 The cell suspension was inoculated in the armpit of the right forelimb at a dose of 0.2 mL....

Embodiment 2

[0037] (1) Take the dry cane of the scutellaria saponifera, cleanly select, cut into sections, and extract with 100% ethanol. The extract obtained after the extract was concentrated and dried was subjected to H-60 ​​macroporous resin chromatography and eluted with 95% ethanol-water as the eluent to obtain the extract. The content of total saponins in the extract was 59.8%. .

[0038] (2) Determination of the IC50 value of the total saponins of Scutellaria saponifera on different tumor cells

[0039] Adherent cells (liver cancer cell Hep G2, breast cancer cell MCF-7, small cell lung cancer SCLC, cervical cancer cell Hela, human colon cancer SW480) IC 50Determination method: take 96-well cell culture plate, add concentration of 3×10 5 cells / mL cell suspension 200 μL, 37°C, 5% CO 2 Adhesive culture in the incubator for 24 hours. After aspirating the culture medium, add 100 μL of serum-free culture medium containing total saponins of Scutellaria saponifera to each well, so tha...

Embodiment 3

[0045] (1) Take the dry cane of the scutellaria saponifera, cleanly select, cut into sections, and extract with 95% ethanol. The extract obtained after the extract was concentrated and dried was chromatographed on DM130 macroporous resin and eluted with 80% ethanol-water as the eluent to obtain the extract. The content of total saponins in the extract was 68.4%. The total saponins of S. saponifera are separated by silica gel column chromatography, and gradient elution is carried out with 70% chloroform-methanol to obtain a refined extract, which is characterized in that the content of saponins A in the refined extract is 92.4%.

[0046] (2) Flow cytometry detection of the apoptosis of Hep G2 cells induced by saponin A

[0047] Huh-7 cells were inoculated into 60mm culture dishes, 100,000 cells per dish, and cultured for 24 hours. Add the medium containing saponin A of saponinus saponin, the concentration is 30, 20, 10 μg·mL -1 , the cells in the control group were not treate...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and particularly relates to application of aspidopterys obcordata hemsl and an extract of aspidopterys obcordata hemsl to the anti-tumor field. The application comprises in vitro application and in vivo application. The aspidopterys obcordata hemsl and the extract of aspidopterys obcordata hemsl can prevent and/or treat tumors like liver cancer, stomach cancer, breast cancer, cervical cancer and colon cancer. The aspidopterys obcordata hemls and the extract of aspidopterys obcordata hemsl can be used for preparing drugs, health-caring products, drinks or food for preventing and/or treating tumors, for example, the aspidopterys obcordata hemsl and the extract of aspidopterys obcordata hemsl can be produced to be orally disintegrating tablets, Pu'er tea soft capsules, aspidopterys obcordata hemsl particles or dissolved medicines, tea bags, aspidopterys obcordata hemsl steroid saponin A oral liquid or tablets. The extract of aspidopterys obcordata hemsl has the good anti-tumor effect, and has no obvious cytotoxicity on the normal tissue cells.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to the anti-tumor application of tunga chinensis and its extract. Background technique [0002] According to the malignant tumor registration data collected by the National Cancer Registry Center in 2016, there were approximately 3.682 million new cases of malignant tumors and 2.229 million deaths in 2013. Lung cancer, gastric cancer, liver cancer, colorectal cancer, female breast cancer, and esophageal cancer account for about 66% of all new cancer cases; lung cancer, liver cancer, gastric cancer, esophageal cancer, and colorectal cancer are the main causes of cancer death, accounting for about 66% of all cancer deaths 70% of cases. Malignant tumors are diseases that seriously threaten human health and social development. According to the latest data released by the World Health Organization, 8.8 million people die from cancer every year in the world, acco...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K31/704A61P35/00A23L33/105A61K135/00
CPCA61P35/00A23L33/105A61K31/704A61K36/185A23V2002/00A61K2236/333A61K2236/55A23V2200/308
Inventor 李宜航李光马国需陈德力陈曦吕亚娜罗云孙晓波
Owner 中国医学科学院药用植物研究所云南分所
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