Large-scale propagation method for dioryctria rubellatetrastichus schoenobii ferriere
A technology for the Spotted Spotted Borer and Spotted Spotted Borer, which is applied in the field of forestry protection, can solve the problems of lack of pests, low drug resistance, pest outbreaks and the like, and achieves the effect of easy operation.
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specific Embodiment approach 1
[0033] Specific embodiment one: the cultivation method of the present embodiment's red pine leaf spot moth nibbler bee comprises the following steps:
[0034] Step 1. Collection and preservation of bee species: collecting and identifying bee species during the pupal stage of S. spp., and storing them under low temperature conditions;
[0035] Step 2. Breeding of bee species: Take out the low-temperature-preserved Korean pine moth wasps by using the finger tube method or the open method, and insert 2 to 30 red pine into each host pupa The wasp is bitten by the spot moth, and the host pupae parasitized by the wasp by the spot moth is put into an incubator or a greenhouse for cultivation.
[0036] Wherein, the second step is to put the host pupa into an aqueous solution of hypochlorous acid with a concentration of ≤25% and soak it before picking up the bees, then take it out, rinse it with distilled water, and dry it.
[0037] The function relationship curve between the developm...
specific Embodiment approach 2
[0040] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step two, the effective accumulated temperature of breeding the red pine leaf spot moth wasp is 1 to 3 days of female bee days, and the humidity is 50 to 75 %. Other steps and parameters are the same as those in Embodiment 1.
[0041] Table 1 shows the developmental starting point temperature and effective accumulated temperature of the red pine moth gnat (with the fruit moth as the host).
[0042] Table 1
[0043]
specific Embodiment approach 3
[0044] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 or 2 is that in step 2, the wasp is a female bee that emerges on the same day or 1 to 2 days after eclosion. Other steps and parameters are the same as those in Embodiment 1 or 2.
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