Sample droplet generation system and method

A generation system and sample liquid technology, applied in biochemical equipment and methods, biochemical instruments, biomass post-processing, etc., to achieve the effects of high utilization rate, authenticity assurance, and increase in the number of effective droplets

Active Publication Date: 2022-03-29
MEDCAPTAIN MEDICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition, due to the inhibitory effect of PCR amplification products on enzyme-catalyzed reactions, the current gene variation detection method of qPCR technology is often powerless for low-abundance tumor gene variation in somatic cells

Method used

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  • Sample droplet generation system and method
  • Sample droplet generation system and method
  • Sample droplet generation system and method

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Experimental program
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Embodiment 1

[0043] Such as Figure 7 , Figure 8 , Figure 9 , Figure 10 The illustrated embodiment is a sample droplet generation system, comprising a needle holder 102, an air pressure tube 103 connected to the needle holder, an air pressure needle 104, a sample droplet generator 101 and a lower part of the sample droplet generator for accommodating the sample droplet generator. The heat conduction container 105; the upper end and the lower end of the heat conduction container are respectively provided with a first sealing cover 151 and a sealing sleeve 152, the first sealing cover is provided with an exhaust port 153, and the exhaust port is provided with a one-way valve 154; the air pressure needle and the sample liquid The droplet generator is connected, and the heat transfer container is provided with droplet carrier oil, such as Figure 7 As shown, the height of the oil surface of the droplet carrier oil is higher than the height of each micro-channel of the sample droplet gene...

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Abstract

The invention discloses a sample droplet generation system and method, comprising a needle holder, an air pressure pipeline connected with the needle holder, an air pressure needle, a sample droplet generator and a heat conduction container for accommodating the lower part of the sample droplet generator; The upper and lower ends of the heat-conducting container are respectively provided with a first sealing cover and a sealing sleeve, the first sealing cover is provided with an exhaust port, and the exhaust port is provided with a one-way valve; the air pressure needle is connected with the sample droplet generator, and the heat-conducting container The droplet carrier oil is arranged in the center, and the lower part of the sample droplet generator is located in the heat conduction container. flow constant pressure pump. The invention has the characteristics of excellent stability and reproducibility, super high sensitivity, extreme precision and low cost.

Description

technical field [0001] The invention relates to the technical field of gene molecular diagnostic reagent equipment, in particular to a sample droplet generation system and method for ensuring that samples in a heat-conducting container are isolated from the outside world. Background technique [0002] At present, fluorescence quantitative PCR (Fluorescence Quantitative Polymerase Chain Reaction, qPCR) has developed into a key routine technology in the field of in vitro molecular diagnosis, which has greatly promoted the development of life science in the field of health care. However, fluorescence quantitative PCR quantification is only relative quantification, and its accuracy and reproducibility still cannot meet the development needs of the current field of in vitro molecular diagnostic systems and biological genetics research. However, in many projects such as liquid biopsy and NIPT, there are some technical bottlenecks. For example, the content of tumor circulating DNA ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12Q1/6806
CPCC12Q1/6806C12Q2565/629
Inventor 王亚琴
Owner MEDCAPTAIN MEDICAL TECH
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