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Hosta ventricosa endophytic fungus metabolites, medical biological activity and application of hosta ventricosa endophytic fungus metabolites

A technology of endophytic fungi and purple flower hosta, applied in the field of microorganisms, can solve the problems of unreported research, long plant growth cycle, and high cost of antibacterial agents

Inactive Publication Date: 2018-10-12
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of extracting antibacterial agents directly from plants is high, and the metabolites produced by plant endophytic fungi can break some of the disadvantages of direct use of plants, including the limitations of long growth cycle of plants themselves, limited yield, and inability to recycle.
According to the "endosymbiosis theory" that the host plant and its microorganisms live together for a long time to produce the same or similar secondary metabolite synthesis pathway, the applicant isolated and screened two kinds of animal pathogenic bacteria from Violet hosta. Active endophytic fungi, related research has not been reported

Method used

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  • Hosta ventricosa endophytic fungus metabolites, medical biological activity and application of hosta ventricosa endophytic fungus metabolites
  • Hosta ventricosa endophytic fungus metabolites, medical biological activity and application of hosta ventricosa endophytic fungus metabolites
  • Hosta ventricosa endophytic fungus metabolites, medical biological activity and application of hosta ventricosa endophytic fungus metabolites

Examples

Experimental program
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Effect test

example 1

[0033] Example 1: Typical isolation of endophytic fungi HoV3 and HoV7 from Hosta japonica

[0034] 1. Collection of plant samples: Fresh and healthy rhizomes of Hosta japonica were collected from Chengdu Campus of Sichuan Agricultural University, rinsed under running water for 12 h, rinsed with 6% sodium hypochlorite for 25 s, blotted the surface liquid with sterile filter paper, rinsed with sterile water for 3 times, absorbed Dry surface liquid for surface disinfection. After surface disinfection, check the cleanliness of the ultra-clean workbench, check the rinsing solution, and screen the sterile tissue blocks with the plant tissue imprinting method.

[0035] 2. Isolation and purification of endophytic fungi: Cut the above-mentioned sterile tissue into small pieces with a diameter of about 0.5 cm on a sterile workbench, place them in PDA medium, and culture them upside down in a constant temperature fungal incubator at 25 °C. When the hyphae of endophytic fungi grow outwar...

Embodiment 2

[0045] Example 2: Antifungal Screening of Endophytic Fungi HoV3 and HoV7 Fermented Broth of Hosta Violet

[0046] 1. Resuscitation and activation of strains: Inoculate the fungi stored in the 4°C refrigerator into PDA medium, place them in a 25°C fungal constant temperature incubator and cultivate them for 7 days, activate them, pick mycelia around the activated colonies and inoculate them into In PDA medium, cultured in a fungal incubator at 25°C for 7 days to restore the normal growth of the fungus.

[0047] 2. Preparation of fermentation products of endophytic fungi HoV3 and HoV7 of Hosta violet: Inoculate the strains on PDA plates and culture at 25 °C for 7 days. On the workbench, use a hole puncher to punch a 6 mm-diameter bacterial cake along the edge of the colony, inoculate it into a 250 mL Erlenmeyer flask containing 100 mL PDB medium; After the body completely covered the liquid surface of the culture solution, the fermentation was terminated, and an uninoculated bo...

Embodiment 3

[0052] Example 3: Screening of active antifungal polar parts of the endophytic fungus HoV7 fermentation liquid of Hosta japonica

[0053] 1. Preparation of different solvent extraction parts of the endophytic fungus HoV7 fermentation broth: After freeze-drying the endophytic fungus HoV7 fermentation broth, disperse with an appropriate amount of distilled water, put the dispersed sample in a separatory funnel, and successively wash with petroleum ether, chloroform, ethyl acetate Esters were extracted 1:1 (v / v), repeated 3 times, the extracts were combined, and the extracts were concentrated and dried in vacuo at 40°C to obtain petroleum ether, chloroform fractions, ethyl acetate fractions, and water fractions (lyophilized).

[0054] 2. Determination of antifungal activity of endophytic fungus HoV7 fermentation broth with different solvent extraction parts: dissolve the above four polar parts with 500 μL DMSO, then dilute to 10 mg / mL with distilled water and add the melted PDA me...

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Abstract

The invention relates to an isolated culture method of two hosta ventricosa endophytic fungi (HoV3 and HoV7) and the inhibiting effect of metabolites of the hosta ventricosa endophytic fungi on animalfungal dermatosis. The fungi are isolated from hosta ventricosa, and the metabolites of the fungi have the good bacteriostatic activity on three types of animal pathogenic bacteria of the sabouraudites lanosus, the microsporum gypseum, and the trichophyton mentagrophytes.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to the application of endophytic fungal metabolites in the preparation of antifungal drugs. Background technique [0002] Hosta ventricosa is a perennial herbaceous plant of the genus Hosta in the family Liliacea. It is mainly distributed in the temperate and subtropical regions of Asia at an altitude of 500-2400 m. It is native to China, Korea and Japan. The whole herb can be used as medicine, and it is a commonly used medicinal material in traditional Mongolian medicine. Among them, the hosta flower is mainly used for boils, snake bites, etc., the hosta leaf is mainly used for sore throat, urination discomfort, burns, etc., and the hosta root eliminates swelling and pain, and removes toxins, etc. . [0003] It is reported that the extracts of hosta flower and root have inhibitory effect on the tested animal pathogenic bacteria. However, the cost of extracting a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61K36/06A61P31/10A61P17/00
CPCA61K36/896A61K36/06A61K2236/19A61K2236/55A61P17/00A61P31/10
Inventor 梁晓霞胡云锴栾尚贤吴强陈楠殷中琼贺常亮吕程张伟袁志祥
Owner SICHUAN AGRI UNIV
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