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Preparation method and application of bovine umbilical cord active ingredient extract

A technology of active ingredients and extracts, which is applied in the field of biological skin care, can solve problems such as allergies, and achieve the effects of simple preparation, low cost, and obvious prospects for industrial application

Active Publication Date: 2021-03-19
北京贝诗丹生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although stem cells and their extracts have a wide range of skin beauty repair functions, for people with sensitive skin, they contain a large amount of heterologous proteins, which are prone to allergies

Method used

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  • Preparation method and application of bovine umbilical cord active ingredient extract
  • Preparation method and application of bovine umbilical cord active ingredient extract
  • Preparation method and application of bovine umbilical cord active ingredient extract

Examples

Experimental program
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preparation example Construction

[0024] Specifically, the invention discloses a method for preparing an extract of bovine umbilical cord active ingredients, which specifically includes:

[0025] Pulverization and homogenization step: wash the fresh bovine umbilical cord with pre-cooled saline, mechanically pulverize it in pre-cooled saline, and homogenize again after washing; for example, homogenize at a temperature of 4°C;

[0026] The crushing and extraction step: the homogenate is crushed by cryo-ultrasound, frozen overnight, centrifuged to take the supernatant, and filtered through a filter membrane to obtain the extract liquid stock solution containing the active ingredient of the bovine umbilical cord.

[0027] Further, there is also a concentration adjustment step: adjusting the stock solution concentration of the extract to 0.1 mg / ml.

[0028] For example, the protein concentration of the prepared bovine umbilical cord active ingredient extract can be measured by ultraviolet spectrophotometer method, ...

Embodiment 1

[0031] Embodiment 1: Preparation of bovine umbilical cord active ingredient extraction stock solution

[0032] Take 250g of fresh bovine umbilical cord, add 500ml pre-cooled normal saline to wash twice, add 500ml pre-cooled normal saline, perform mechanical crushing at 4°C, add 500ml pre-cooled normal saline to wash twice, add 1000ml pre-cooled normal saline, Homogenize at 4°C, sonicate at 4°C, freeze overnight at -80°C, centrifuge at 4°C (3600rpm×10min), take the supernatant, and filter with a 0.22μ filter membrane to obtain the bovine umbilical cord active ingredient extract stock solution.

Embodiment 2

[0033] Embodiment 2: the preparation of bovine umbilical cord active ingredient extract

[0034] Initialize the UV spectrophotometer, preheat it for 30 minutes, and use normal saline as a blank for calibration. Pour the prepared bovine umbilical cord active ingredient extraction stock solution into a quartz cup and place it in the sample cell. Read the samples at A260 and A280 respectively. Absorbance value (A280=0.418, A260=0.314 in this example), the protein concentration of the stock solution was calculated according to the formula=1.45×A280−0.74×A260=0.374mg / ml.

[0035] According to the calculated protein concentration, the bovine umbilical cord active ingredient extraction stock solution is diluted, and its final concentration is determined to be 0.1 mg / ml, which is the bovine umbilical cord active ingredient extraction solution. Taking the concentration in this example as an example, the dilution method is to take 100ml of the bovine umbilical cord active ingredient ext...

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Abstract

The invention discloses a preparation method of a bovine umbilical cord active ingredient extracting solution and an application of the extracting solution in human skin repair. According to the method, a supernatant containing bovine umbilical cord active ingredients is prepared with a method including cleaning, crushing, homogenizing and crushing and taking and filtering of the supernatant, andthe supernatant is adjusted to a stock solution with proper concentration. Glutathione is added to the stock solution in a certain proportion to increase the antioxidant function of skin, dipotassiumglycyrrhizinate is added to reduce the allergic reaction of skin, so that a human skin repair product is prepared and applied to repair of human skin. Compared with other cosmetic products, the extracting solution has the advantages that the problem of stem cell stemness loss generally exists in ordinary stem cell products is solved, all components of wharton's jelly in bovine umbilical cord are reserved, meanwhile, antioxidant and antiallergic components are added and have the synergistic effect with the bovine umbilical cord active ingredients, nutrition and repair of skin are better facilitated, and the extracting solution is low in cost and simple to prepare and has remarkable industrial application prospects.

Description

technical field [0001] The invention belongs to the field of biological skin care, and in particular relates to a preparation technology of an active ingredient extract of bovine umbilical cord, a skin care product prepared by using the active ingredient of bovine umbilical cord, and an application of the skin care product in beauty repair of human skin. Background technique [0002] The development of medicine and biotechnology, especially the discovery of stem cells has injected new vitality into human skin repair and anti-aging technology. Skin is one of the fastest tissues for body renewal and tissue regeneration. Studies have shown that human skin contains at least 6 kinds of adult stem cell populations, which are constantly undergoing directed differentiation to maintain the homeostasis of skin cells. At the same time, skin stem cells can contribute to the surrounding microenvironment. It secretes a large number of cytokines and various repair proteins, such as EGF, bF...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/98A61Q19/00
CPCA61K8/982A61K2800/805A61K2800/82A61Q19/00
Inventor 修冰水
Owner 北京贝诗丹生物科技有限公司
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