A kind of Pasteurella avian capsular polysaccharide-protein conjugate vaccine and its preparation method

Abstract

Belonging to the technical field of biology, the invention in particular relates to a pastevula mulfocida capsular polysaccharide-protein conjugate vaccine and a preparation method thereof. The pastevula mulfocida capsular polysaccharide-protein conjugate vaccine provided by the invention is formed by connection of pastevula mulfocida capsular polysaccharide and diphtheria toxoid carrier protein through nano-microspheres. The combination of pastevula mulfocida capsular polysaccharide with the carrier protein diphtheria toxoid enhances the immune efficacy of the vaccine, and also reaches a cross protection effect on the infection of homologous strains and heterologous strains. The preparation method of the pastevula mulfocida capsular polysaccharide-protein conjugate vaccine provided by theinvention has the characteristics of simple operation and cost saving, and has good application prospect.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a Pasteurella avian capsular polysaccharide-protein conjugate vaccine and a preparation method thereof. Background technique

[0002] Pasteurella multocida, referred to as Pasteurella for short, is a short Gram-negative bacillus with capsules that can infect many animals such as livestock, poultry and wild birds. Clinical studies have shown that fowl cholera is a contagious disease mainly caused by serotype A:1, A:3 or A:4 Pasteurella, which causes serious economic losses to the poultry industry.

[0003] Chinese patent application CN105288607A discloses a preparation method of an inactivated vaccine for avian pasteurellosis. The inactivated vaccine for avian pasteurellosis prepared by the method is effective in immunizing rabbits. However, in the process of using Among them, this inactivated vaccine has a short action time in the body and needs multiple vaccinations, wh...

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