Methylobacterium strain and application thereof

A technology of Methylobacter and Methylobacter extortion, applied in the field of microorganisms, can solve the problems of scarcity of biological seed coating agents, chemical residues, and sudden pollution of water sources, so as to achieve the effect of not easily losing vitality and reducing the use of chemical fertilizers and pesticides.

Inactive Publication Date: 2018-11-06
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Abstract
  • Description
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Problems solved by technology

[0006] At present, most of the seed coating agents use chemical preparations, which will cause ch...

Method used

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  • Methylobacterium strain and application thereof
  • Methylobacterium strain and application thereof
  • Methylobacterium strain and application thereof

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Experimental program
Comparison scheme
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Embodiment Construction

[0023] Specific embodiments of the present invention will be described in detail below.

[0024] 1. Isolation and screening of Methylobacterium strains

[0025] The samples were collected from sweet potato leaves from farmland in Naling Township, Daxin County, Chongzuo City, Guangxi Province.

[0026] Weigh 1g of sweet potato leaves, add them to sterilized 100mL Tween water with a concentration of 0.1%, crush the leaves with a hand-held crusher, and dilute the leaf liquid gradient to 10 -2 、10 -3 concentration, and then spread it on AMS solid inorganic salt medium, and culture it upside down in a 28°C constant temperature incubator for 5 days. Inverted culture in medium for 5 days, then observe the colony type, and observe the cell type with an optical microscope (1000X).

[0027] The formula of AMS solid inorganic salt medium is: anhydrous dipotassium hydrogen phosphate 700mg, anhydrous potassium dihydrogen phosphate 540mg, magnesium sulfate heptahydrate 1g, anhydrous ammo...

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Abstract

The invention belongs to the technical field of microorganisms and particularly relates to a Methylobacterium strain and application thereof. The Methylobacterium strain has taxonomic name, Methylobacterium extorquens, collected in China General Microbiological Culture Collection Center on May, 21, 2018 under CGMCC No. 15777. Application of the Methylobacterium strain in the preparation of microbial fertilizers is also provided. Further, a microbial seed coating agent made with the Methylobacterium strain is provided. The Methylobacterium strain and application thereof have the advantages thatthe consumption of chemical fertilizers and pesticides in corn planting is decreased so that high yield is achieved; the Methylobacterium strain is more important to seeds that are inactivated easily.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a methylobacterium strain and its application. Background technique [0002] Corn is the second largest crop in the world, and my country is the second largest producer of corn in the world, with an annual output of more than 67.5 million tons of corn. Corn is one of the main food crops with the largest demand and wide application fields in my country. [0003] In agricultural production, the extensive use of chemical fertilizers and pesticides has caused problems such as soil degradation, crop quality and yield decline, and ecological environment deterioration, threatening my country's food and environmental security. Therefore, using the physiological properties of microorganisms to provide the nutrients needed for crop growth, or to improve the granularity of the soil, or to resist pests and diseases in the environment, the position in the production of saf...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 王科晶蒋先芝
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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