Biomarker for detecting idiopathic inflammatory myopathies and application of biomarker

An idiopathic and useful technology, applied in the field of biomarkers for the detection of polymyositis and/or dermatomyositis, can solve the problems of complex clinical manifestations, difficulty in early diagnosis, early treatment, and misdiagnosis of patients.

Active Publication Date: 2018-11-06
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different types of idiopathic inflammatory myopathy can involve different target organs such as skin, lung, and muscle, so the clinical manifestations are more complicated, and it is difficult to achieve early diagnosis and early treatment. Malnourished patients have similar pathological changes, so some patients are easy to be misdiagnosed

Method used

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  • Biomarker for detecting idiopathic inflammatory myopathies and application of biomarker
  • Biomarker for detecting idiopathic inflammatory myopathies and application of biomarker
  • Biomarker for detecting idiopathic inflammatory myopathies and application of biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 High-density protein chip screening PM / DM related autoantibodies

[0029] The N-terminal of all protein antigens on the high-density protein chip has a GST tag for protein purification. First, use rabbit anti-GST monoclonal antibody to hybridize with chip protein to verify chip quality. Then, 40 PM / DM patient sera, 30 autoimmune disease control sera, and 20 healthy control sera were selected for hybridization with 90 high-density protein chips, and candidate PM / DM-related autoantigens were identified through signal acquisition and data analysis.

[0030] 1.1 Extraction of high-density protein chip scanning data

[0031] The GenePix 4000B chip scanner was used to scan the high-density protein chip, and after obtaining the grayscale image in TIFF format, according to the operation manual of the GenePix Pro 6.0 software, and according to the high-density protein chip loading matrix parameters, the analysis software automatically completed each protein point. Se...

Embodiment 2

[0052] Example 2 Construction of PM / DM Related Autoantibody Target Antigen Protein Chip and Serum Screening Verification

[0053] Due to differences in genetic background and environmental factors in patients with autoimmune diseases, the types of autoantibodies produced by individuals with the same autoimmune disease are quite different. In order to confirm whether the PM / DM-related autoantibodies screened from small samples by high-density protein chips have clinical significance and application value, and the sensitivity and specificity of these autoantibodies to the diagnosis of PM / DM patients, this study will use Example 1 PM / DM-related autoantibody target antigens screened by high-density protein chips were prepared into PM / DM-related autoantibody target antigen protein chips for clinical expansion sample verification. Serum samples used for PM / DM-related autoantibody target antigen protein chips for clinical large-scale validation testing included 397 PM / DM patients, 20...

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Abstract

The invention discloses a signal recognition particle 19, namely SRP 19, or application of a fragment of the signal recognition particle 19 in preparation of a reagent for diagnosing idiopathic inflammatory myopathies. A high-density protein chip technology is adopted for screening target antigens of PM/DM-associated autoantibodies to determine 91 PM/DM-associated autoantibodies. A PM/DM-associated autoantibody target antigen chip is established by and prepared from the target antigens corresponding to the 91 PM/DM-associated autoantibodies, and clinical enlarged sample verification is conducted. The result shows that the positive rate of an anti-SRP19 antibody in a PM group is 19.00%, and is higher than that of all control groups by 2.67% (P is equal to 3.26*10<-7>, and the result difference has significance; and the positive rate of the anti-SRP19 antibody in a DM group is 7.41%, and is higher than that of all the control groups by 2.67% (P is equal to 0.80*10<-2>), and the result difference has significance, the positive rate of the anti-SRP19 in a PM/DM group is 10.33%, and is higher than that of all the control groups by 2.67% (P is equal to 0.09*10<-3>), and the result difference has significance. The test shows that the anti-SRP19 antibody can be used as a diagnosis and detection marker for idiopathic inflammatory myopathies.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a biomarker for detection of polymyositis and / or dermatomyositis and application thereof. Background technique [0002] Idiopathic inflammatory myopathies (IIMs) are heterogeneous diseases characterized by proximal symmetrical muscle weakness and multiple organ involvement, mainly including polymyositis (PM), dermatomyositis ( dermatomyositis (DM), immune-mediated necrotizing myopathy (IMNM), sporadic inclusion body myositis (sIBM), juvenile idiopathic myositis (JIM), etc. clinical subtype. PM / DM is an autoimmune disease of striated muscle nonsuppurative inflammatory myopathy. Its clinical features are elevated serum muscle enzymes, myogenic EMG changes, inflammation and degenerative changes in muscle tissues such as limb girdle muscles, neck muscles, and pharyngeal muscles, resulting in symmetrical muscle weakness and a certain degree of muscle atrophy. Involve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/532G01N33/577
CPCG01N33/532G01N33/54313G01N33/577
Inventor 李永哲胡朝军吴琳李柳冰刘晨曦张奉春
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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