Regulation effect of TRPV1 on MAP addiction
A function and addiction technology, applied in the medical field, can solve the problems of addiction treatment dilemma and less research on MAP addiction mechanism.
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[0010] The present invention will be described in detail below in combination with specific embodiments.
[0011] The use of TRPV1 antagonists and TRPV1 knockout mice has well documented that TRPV1 is involved in the regulation of MAP addiction and is achieved by altering presynaptic dopamine (dopamine, DA) release in key brain regions, a pathway that alters the cause of DA release is involved in the MAPK signaling pathway mediated through the DA receptor. In addition, TRPV1 also directly regulates MAP. The present invention utilizes optimized animal and cell models, adopts electrophysiological techniques and various molecular biological techniques to clarify its exact molecular mechanism, thereby providing reliable clues for the research on the treatment of addiction of MAP.
[0012] Experiment 1. Using intracerebral microinjection of TRPV1 antagonists to confirm that its regulatory effect on MAP addiction is specific to brain regions;
[0013] 1. Using C57BL / 6J male mice, ...
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