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Open tissue culture method for Huangzhou radish

A technology of tissue culture and tissue culture medium, which is applied in the field of plant tissue culture, can solve the problems of high cost, cumbersome technology, and difficulty in completely solving medium pollution, and achieve the effect of simplifying the tissue culture process and saving costs

Inactive Publication Date: 2018-11-23
HUANGGANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional tissue culture is carried out in a sterile environment, and its disadvantages are that it is difficult to completely solve the contamination of the medium, the cost is too high, the large-scale technology is immature and the technology is cumbersome.

Method used

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  • Open tissue culture method for Huangzhou radish

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Experimental program
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Effect test

Embodiment 1

[0012] (1) Disinfection of the culture container: Soak a disposable transparent PP plastic container with a cover in 0.1% sodium hypochlorite solution for 16 hours, and dry it for later use.

[0013] (2) MS medium preparation: according to the conventional MS medium components, that is, 1L medium contains 25g of sucrose, 7g of agar powder, KNO 3 1900mg, NH 4 NO 3 1650mg, MgSO 4 ·7H 2 O 370mg, KH 2 PO 4 170mg, CaCl 2 2H 2 O 440mg, MnSO 4 4H 2 O 22.3mg, ZnSO 4 ·7H 2 O 8.6mg, H 3 BO 3 6.2mg, KI 0.83mg, Na 2 MoO 4 2H 2 O 0.25mg, CuSO 4 ·5H 2 O 0.025mg, CoCl 2 ·6H 2 O 0.025mg, Na 2 ·EDTA·2H 2 O 37.3mg, FeSO 4 ·7H 2 O27.8mg, glycine 2.0mg, pyridoxine hydrochloride V B6 0.5mg, ammonium sulfate hydrochloride V B1 0.1mg, 0.5mg of niacin, and 100mg of inositol were used to prepare basal medium, heated until the agar powder was completely melted, and continued to boil for 5 minutes.

[0014] (3) Open medium preparation: Cool the above MS medium to 55°C, th...

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Abstract

The invention discloses an open tissue culture method for a Huangzhou radish, and belongs to the technical field of plant tissue culture. The open tissue culture method for the Huangzhou radish comprises the following steps that a culture medium is prepared, then is packaged into disinfected culture vessels in a split mode, is cooled and solidified and then is inoculated with test-tube plantlets of the Huangzhou radish, and the test-tube plantlets are cultured in an open environment, wherein the culture medium, in volume of 1L of culture medium, is prepared by adding 25 g of cane sugar, 7 g ofagar powder, 2 mg of 6BA, 0.02 mg of NAA and 500-2000 mg of sodium hypochlorite on the basis of an MS culture medium body, and high-temperature and high-pressure sterilization is not need in the preparation process of the culture medium. Compared with a traditional tissue culture technology requiring high-temperature and high-pressure sterilization, the open tissue culture method for the Huangzhou radish has the advantages that the growth speed and the average contamination rate of the test tube plantlets in the two methods have no obvious difference; and a strict aseptic environment is not needed, the tissue culture cost of the Huangzhou radish can be greatly lowered, and the development of the industry of the Huangzhou radish of a product of geographical indication in Dabie Mountain Area is beneficially promoted.

Description

technical field [0001] The invention relates to plant tissue culture technology in the field of tissue culture agriculture and forestry biotechnology, in particular to an open tissue culture method for Huangzhou radish without high temperature and high pressure sterilization. Background technique [0002] Huangzhou radish is a local characteristic vegetable variety in the Dabie Mountains of Huanggang City, Hubei Province. It has been recorded in history since the Eastern Han Dynasty and has a long history of cultivation. On September 19, 2008, Huangzhou radish was approved as a geographical indication product by Announcement No. 103 of the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. Huangzhou radish is sweet, crispy and delicious when eaten raw, and it tastes good when it is cooked. . However, Huangzhou radish is easily affected by pollen from other radish varieties and even other cruciferous plants during the...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李世升李竟才方元平
Owner HUANGGANG NORMAL UNIV
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