Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of mtat-hegf-kcd47 fusion protein and its construction method and application

A mtat-hegf-kcd47, fusion protein technology, applied in the field of construction of mTAT-hEGF-kCD47 fusion protein, mTAT-hEGF-kCD47 fusion protein, can solve the problems of immune system clearance, macromolecular protein cannot actively penetrate the epidermis, etc. , to achieve the effect of long-term action and low cost

Active Publication Date: 2021-08-03
XIAN MEDICAL UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The first object of the present invention is to provide a mTAT-hEGF-kCD47 fusion protein, which solves the problem that macromolecular proteins cannot actively penetrate the epidermis and are easily cleared by the immune system in the skin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of mtat-hegf-kcd47 fusion protein and its construction method and application
  • A kind of mtat-hegf-kcd47 fusion protein and its construction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

An mTAT-hEGF-kCD47 fusion protein disclosed in the present invention is expressed by fusion of mTAT, kCD47 and hEGF, and the whole sequence of mTAT-hEGF-kCD47 is designed, which is artificially synthesized and then connected into the pTrcHisB expression vector to successfully construct mTAT-hEGF ‑kCD47 recombinant expression plasmid vector, through expression and purification to obtain biologically active mTAT‑hEGF‑kCD47 fusion protein, has the advantages of low cost, high activity, and long-lasting action. The mTAT-hEGF-kCD47 fusion protein of the present invention not only retains the original penetration of TAT and the effect of CD47 on inhibiting non-specific immune responses, but also has almost no effect on the activity of the foreign protein hEGF, so that it can be used in the preparation of products that promote epidermal proliferation. It has broad prospects in medicine and skin care products.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to an mTAT-hEGF-kCD47 fusion protein, and also relates to a construction method and application of the mTAT-hEGF-kCD47 fusion protein. Background technique [0002] Epidermal growth factor (EGF) has various functions such as promoting the proliferation of epidermal cells. Human epidermal growth factor (hEGF) is a single-chain polypeptide composed of 53 amino acid residues, its molecular weight is 6kDa, and its isoelectric point pI is 4.2. The hEGF molecule has high stability and can withstand the effects of acid, alkali, heat and pepsin and trypsin. A large number of studies have confirmed that hEGF has the ability to stimulate the proliferation and differentiation of epidermal cells. It can accelerate the process of new cells replacing aging cells by promoting the synthesis of DNA in epidermal cells, increase the uptake of other endogenous growth factors by ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/70A61K38/18A61K38/17A61K47/42A61K8/64A61P17/00A61P17/02A61Q19/00
CPCA61K8/64A61K47/42A61Q19/00A61P17/00A61P17/02C07K14/485C07K14/70503
Inventor 苟兴春高兴春胡峰瑞米亚静
Owner XIAN MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com