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Preparation and application of a photoelectrochemical competitive immune sensor for alpha-fetoprotein based on dual-sensitization structure

A kind of alpha-fetoprotein, photoelectrochemical technology

Inactive Publication Date: 2019-05-28
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most traditional PEC immunoassay methods only introduce a single photoactive material, which has certain limitations in the actual application process, such as the limited range of light absorption, the inability to effectively prevent the recombination of photogenerated electron-hole pairs, and the photocurrent conversion efficiency. inferior

Method used

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  • Preparation and application of a photoelectrochemical competitive immune sensor for alpha-fetoprotein based on dual-sensitization structure
  • Preparation and application of a photoelectrochemical competitive immune sensor for alpha-fetoprotein based on dual-sensitization structure
  • Preparation and application of a photoelectrochemical competitive immune sensor for alpha-fetoprotein based on dual-sensitization structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of a photoelectrochemical competitive immunosensor for alpha-fetoprotein based on a double-sensitized structure (such as figure 1 shown):

[0026] (1) Pretreatment of glassy carbon electrode (GCE): GCE is first mechanically polished and polished on the suede covered with alumina powder, washed with secondary water to remove residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is cleaned, and finally Wash thoroughly with ethanol, dilute acid and water in sequence;

[0027] (2) Add 3 μL of Bi at a concentration of 3 mg / mL dropwise 2 S 3 The nanosheet suspension is placed on the surface of a clean glassy carbon electrode, dried under an infrared lamp, and cooled to room temperature;

[0028] (3) Drop-coat 3 μL of ionic liquid (IL) with a concentration of 1 mg / mL on the surface of the above electrode, dry it under an infrared lamp, and cool to room temperature;

[0029] (4) Soak the modified electrode in a mixtur...

Embodiment 2

[0034] An application of a photoelectrochemical competitive immunosensor for alpha-fetoprotein based on a dual-sensitization structure, the steps are as follows:

[0035] (1) Measured by a three-electrode system, with the Bi prepared in Example 1 2 S 3 / IL / AFP / Ab-TiO 2 -PEI modified electrode is the working electrode, Ag / AgCl is the reference electrode, and the platinum wire electrode is the counter electrode. The photoelectrochemical workstation is used for detection. The voltage is set to 0.1V, and the lamp is switched on and off every 10s. The monochromatic light emitted by the xenon lamp The excitation light source is filtered by a monochromator before use;

[0036] (2) In PBS buffer solution with pH 7.0, detect 1×10 by photoelectrochemical workstation -6 ng / mL-10 ng / mL series of alpha-fetoprotein standard solutions with different concentrations, by recording the different current signals before and after switching the lamp, draw the working curve. Figure 2 A is diffe...

Embodiment 3

[0039] Bi described in Example 1 2 S 3 Preparation of nanosheets:

[0040] 0.00375 mol Bi(NO 3 ) 3 · 5H 2 O (purchased from Shenyang Xinguang Chemical Factory) was dissolved in 25 mL ethanol and stirred for 20 min to obtain solution A; 0.005625 mol Na 2 Dissolve S in 30 mL deionized water and stir for 10 min to prepare solution B. Add solution B slowly to solution A; when a large amount of black suspension is produced, add 0.032 mol urea (CO(NH 2 ) 2 ) and 20 mL of deionized water and sealed the prepared mixed solution in an autoclave, and kept it at 180°C for 12h; subsequently, filtered the mixed solution to obtain a black solid product, washed it with deionized water and dried in air; Finally, the above-prepared Bi 2 S 3 The nanosheets were dissolved in N, N-dimethylformamide (DMF) and diluted to prepare solutions with different concentrations.

[0041] TiO as described in Example 1 2 Preparation of mesogens:

[0042] First, dissolve and disperse 0.25 g of polyvin...

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Abstract

The invention discloses preparation and application of an alpha-fetoprotein photoelectrochemical competitive immunosensor based on a double-sensitized structure. A sensing interface is constructed byusing titanium dioxide mesocrystals as a probe substrate and immobilizing an alpha-fetoprotein antibody (Ab), and using bismuth sulfide as an electrode substrate and immobilizing an alpha-fetoproteinantigen; the sensor with the double-sensitized structure is constructed by means of specific binding between an antigen and the antibody. An electrode modified with the alpha-fetoprotein antigen is placed in a mixed solution containing different concentrations of free antigen and a certain amount of labeled probes, and the free target antigen competes with the immobilized antigen for bonding to the labeled probes. Under the illumination condition, the electrode bonded with the probes can generate photocurrent with certain intensity, and the amplification of a current signal is realized by means of the double-sensitized structure. Furthermore, as the concentration of the free target material increases, the number of the probes bonded to the immobilized antigen decreases, and the photocurrent intensity is also reduced. Based on the phenomenon, a photoelectric analysis method for alpha-fetoprotein can be established.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and specifically relates to the preparation and application of an alpha-fetoprotein photoelectrochemical competitive immunity sensor based on a double-sensitization structure. Background technique [0002] Photoelectrochemical (PEC) detection uses light as the excitation signal and photocurrent as the detection signal. By using different forms of energy as the excitation signal and detection signal, the excitation and detection signals do not interfere with each other, so the background signal is low and can be obtain higher sensitivity. In the construction process of photoelectrochemical sensors, the choice of photosensitive materials is crucial to the response of the signal. Among the currently used materials, TiO 2 Nanomaterials are ideal materials for photocatalytic and photoelectrochemical sensors because of their unique photocatalytic activity, n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/308G01N27/3278
Inventor 戴宏陈妍洁郑祥钦高利红衣欢林丹玫
Owner FUJIAN NORMAL UNIV
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