Method for identifying reality and purity of pepper male sterile three-line mating hybrids based on InDel (insertion-deletion) molecular markers

An identification method and male sterility technology, which can be applied in biochemical equipment and methods, recombinant DNA technology, and microbial assay/inspection, etc., can solve problems such as inability to determine the authenticity of seeds, and achieve the effect of narrowing the scope and widening application prospects.

Active Publication Date: 2018-11-27
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In pepper, especially in the field of processed pepper, there is no complete and widely applicable method for quickly identifying the authenticity of male sterile three-line hybrids of pepper
And the current purity identification in peppers is not combined with the authenticity identification, even if the purity identification is carried out, it is still impossible to determine the authenticity of the identified seeds

Method used

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  • Method for identifying reality and purity of pepper male sterile three-line mating hybrids based on InDel (insertion-deletion) molecular markers
  • Method for identifying reality and purity of pepper male sterile three-line mating hybrids based on InDel (insertion-deletion) molecular markers
  • Method for identifying reality and purity of pepper male sterile three-line mating hybrids based on InDel (insertion-deletion) molecular markers

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Material preparation and cultivation for authenticity identification of processed pepper hybrids

[0031] 1. Material preparation

[0032] The material for authenticity and purity identification of processed pepper hybrids in the present invention requires the hybrids to be identified and their parental materials. There are 4 hybrid species to be identified in the present invention, including 3 types of processed peppers, including cleavage pepper, sweet pigment pepper and cluster pepper, as shown in Table 1.

[0033] Table 1 Processed pepper hybrids and parent materials to be identified for authenticity

[0034]

[0035] 2. Material cultivation

[0036] (1) Take 50 full seeds each of the hybrids of the three-line processing pepper and its parent parent, soak the seeds in a warm soup at 50°C for 30 minutes, and soak them at room temperature for 8 hours.

[0037] (2) In a petri dish, place 2 layers of filter paper on the bottom and 1 layer of filter paper on the u...

Embodiment 2

[0093]The method for identifying the authenticity and purity of the processed pepper male sterile three-line matching hybrid based on the InDel molecular marker provided by the present invention specifically includes the following steps:

[0094] (1) the processing type pepper hybrid to be identified and the parent are cultivated, until the sprout is cultivated;

[0095] Take 50 plump seeds of the hybrids of the three-line processing pepper and its parental parent, soak the seeds in warm soup at 50 °C for 30 minutes, and soak them at room temperature for 8 hours. In a petri dish, place 2 layers of filter paper on the bottom and 1 layer of filter paper on the upper layer, and wet the filter paper with distilled water. Place the soaked seeds on the filter paper, and place them in a 28°C incubator for 7-10 days. During this period, rinse them with distilled water 3 times in the morning and evening to keep the filter paper moist, but pay attention that the distilled water cannot c...

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Abstract

The invention provides a method for identifying reality and purity of pepper male sterile three-line mating hybrids based on InDel (insertion-deletion) molecular markers. The method includes the steps: culturing the pepper hybrids and parents to a bud and seedling stage; extracting DNA (deoxyribonucleic acid); screening the hybrids and the parents by core primers. Primers of co-dominant markers can serve as molecular markers for identifying the reality of the hybrids, and co-dominant markers of the pepper hybrids and the parents continues to be screened by combining a secondary core primer library until the co-dominant markers are screened out if the co-dominant markers of the pepper hybrids and the parents cannot be screened out in a core primer library. According to the method, a systemcapable of rapidly and simultaneously identifying the reality and the purity of the pepper male sterile three-line mating hybrids is built and cannot be limited by time and seasons, indoor rapid identification of the reality and the purity of the hybrids is achieved, and molecular biology bases are provided for protection and market supervision of the pepper male sterile three-line mating hybrids.

Description

technical field [0001] The invention belongs to the technical field of capsicum breeding, and in particular relates to a method for identifying the authenticity and purity of capsicum male sterile three-line matching hybrids based on InDel molecular markers. Background technique [0002] Processed pepper is an important type of pepper varieties, and it is an important source of seasoning and pigment extraction. my country's planting area is the second in the world, second only to India. Compared with conventional varieties, male sterile three-line matching hybrids of pepper have obvious advantages such as uniformity, high yield and high quality, and strong resistance, and their planting area and demand are constantly increasing. The varieties of processed peppers are updated very quickly. Not only are there many approved varieties, but also the similarities between different varieties are very large, and the differences are very small. It is generally difficult to accurately...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 林多王莉月王辉马静杨延杰代佳汐刘建萍
Owner QINGDAO AGRI UNIV
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