Concentration response type biochemical multi-component online analyzer

A multi-component and analyzer technology, which is applied in the analysis of materials, material analysis through electromagnetic means, instruments, etc., can solve the problem of prolonging the detection cycle, inability to guide and control the fermentation process, and detection data that cannot reflect changes in component concentrations in time, etc. problem, to achieve the effect of compact structure and convenient mass production

Active Publication Date: 2018-11-30
NANJING UNIV OF TECH
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  • Claims
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Problems solved by technology

Most of the component concentration analysis instruments currently on the market use off-line sampling, dilution requires manual sampling and complex pretreatment for off-line detection of low-concentration component concentrations, while the component concentrations in fermentation systems are often much higher Due to the detection linear range of these instruments, it is necessary to dilute the sample for offline detection, whic

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  • Concentration response type biochemical multi-component online analyzer
  • Concentration response type biochemical multi-component online analyzer
  • Concentration response type biochemical multi-component online analyzer

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Embodiment 1

[0037] Such as Figures 1 to 5 As shown, a concentration-responsive biochemical multi-component online analyzer includes a multi-component concentration detection module, a precise sample injection robot arm, a multi-channel data acquisition module, an automatic sampling module and a multi-channel flow path structure.

[0038] The specific connection of each module is as follows:

[0039] The internal connection of the multi-component sensing and detection module: the centers of all functional pools (detection pool, cleaning pool, waste liquid pool, sampling pool, standard sample pool) are fixed on a central circle in the center of the disc. The cavities of the detection pools A, B, and C are all designed with inverted round tables, and different biosensing electrodes are placed inside. The top of the pool body is made of a sealed soft plug made of silica gel, and a rectangular data acquisition interface is set on the soft plug (for The biosensing electrode is connected to th...

Embodiment 2

[0048] Step 1: Multi-component sensing module structure design. Design base support disc size: 25cm in diameter and 3cm in thickness; fix detection pool A, detection pool B, detection pool C, cleaning pool, waste liquid pool, sampling pool, and standard sample clockwise along the edge of the base disc pool. The cavities of the multi-component detection pools A, B, and C are all designed with inverted round tables. The volume of the cavity is 5ml. The material is made of organic glass. Its advantages are highly transparent, and the internal conditions of the sample pool can be observed. It is corrosion-resistant and moisture-resistant. Good insulation performance, will not affect the electrode work. Different biosensing electrodes are placed inside the cell body, a magnetic stirrer is fixed at the lower part of the cell body, the top of the cell body is sealed with a sealing cork made of silica gel, and a rectangular data acquisition interface is set on the cork (for biosensin...

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Abstract

The invention discloses a concentration response type biochemical multi-component online analyzer. The concentration response type biochemical multi-component online analyzer comprises a multi-component concentration detection module, a precise sampling mechanical arm, a multi-channel data acquisition module, an automatic sampling module and a multi-channel flow path structure; the multi-componentconcentration detection module is composed of a detection pool, a sample pool, a sampling pool, a cleaning pool and a waste liquid pool, and can realize online detection on multi-component concentration; the sampling mechanical arm is composed of a two-freedom-degree mechanism arm, a precision syringe and a sample injection needle, and realizes precise locating sampling control on the detection pool; the multi-channel data acquisition module is composed of an embedded development board and a touch screen, and realizes online detection, display and storage on the multi-component concentration;the automatic sampling module is composed of a precision membrane sampler, a miniature vacuum diaphragm pump and a stop valve; and the four parts are linked and assembled through a multi-channel flowpath integrated structure. According to the concentration response type biochemical multi-component online analyzer disclosed by the invention, online real-time detection on multiple substrates, keyintermediate metabolites and product concentrations in a microbial fermentation process can be realized; the structure is compact; and batch production is conveniently carried out.

Description

technical field [0001] The invention belongs to the interdisciplinary field of biosensing technology and industrial automation technology, and specifically relates to an on-line multi-component concentration analyzer for microbial fermentation processes based on a biosensing electrode and capable of responding to various component concentrations. Background technique [0002] The fermentation process mainly involves the growth and metabolism of microorganisms. The substrates, key intermediate metabolites, product types and concentrations and interactions in the fermentation system will all affect the reaction rate, product yield and cell-to-metabolism pathways in the microbial fermentation process. choice has a significant impact. The purpose of regulating the fermentation process is to adjust the environment and other factors to the most suitable state for the growth of microorganisms. During the regulation process, it is necessary to understand the relevant information of ...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 薄翠梅金万勤吴昊储震宇王轶卿王晓荣
Owner NANJING UNIV OF TECH
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