A kind of color-preserving fixative for anatomy teaching specimen and preparation method thereof
A color fixation and specimen technology, applied in the field of specimen fixatives, can solve the problems of irritant, toxic, flammable and explosive, affecting the fidelity of specimens, and discoloring specimens, achieving remarkable environmental protection properties, and improving production quality and production efficiency. , the effect of long color retention time
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[0026] A method for preparing a color-preserving fixative for an anatomy teaching specimen, comprising the following steps:
[0027] (1) Preparation of citrus fruit peel extract
[0028]Take the peel of the citrus fruit, wash and dry it, put it in a crusher and break it into a slurry, and then add 0.3-0.7% of its mass cellulase and 0.2-0.4% of its mass to the slurry. Glue enzyme, after stirring evenly, carry out enzymolysis treatment at 40-50°C for 1.5-2.5h. During the enzymolysis treatment, perform ultrasonic treatment for 20-30S every 30min, and then, under constant stirring, Add NaOH solution to the obtained enzymatic hydrolysis product until the pH of the obtained mixture is 10-11, then carry out vacuum filtration, take the filtrate, and under the condition of constant stirring, assist in the ultrasonic vibration treatment with a frequency of 55-65KHz, add to the filtrate Add an acid-base regulator acetic acid solution or phosphoric acid solution to adjust the pH of the f...
Embodiment 1
[0037] A color-preserving fixative for anatomy teaching specimens, consisting of the following components in parts by weight: 2.5 parts of sodium lauryl sulfate, 12 parts of urea, 0.5 parts of potassium sorbate, 0.3 parts of citric acid, and 1,2-propylene glycol 20 parts, 0.6 parts of sodium tripolyphosphate, 0.5 parts of ascorbic acid, 0.2 parts of L-cysteine, 1 part of citrus fruit peel extract, 1.2 parts of tea residue extract, 65 parts of distilled water.
[0038] A method for preparing a color-preserving fixative for an anatomy teaching specimen, comprising the following steps:
[0039] (1) Preparation of citrus fruit peel extract
[0040] Get the pericarp of citrus fruit, wash and dry, place in a crusher and break into a slurry, after that, add 0.6% of its mass of cellulase and 0.3% of its mass of pectinase to the slurry, stir After uniformity, carry out enzymolysis treatment at 45°C for 2 hours. During the enzymolysis treatment, perform ultrasonic treatment for 25 seco...
Embodiment 2
[0048] A color-preserving fixative for anatomy teaching specimens, consisting of the following components in parts by weight: 2 parts of sodium lauryl sulfate, 15 parts of urea, 0.3 parts of potassium sorbate, 0.2 parts of citric acid, and 1,2-propylene glycol 18 parts, 0.7 parts of sodium tripolyphosphate, 0.4 parts of ascorbic acid, 0.1 parts of L-cysteine, 1.3 parts of citrus fruit peel extract, 1 part of tea residue extract, 60 parts of distilled water.
[0049] A method for preparing a color-preserving fixative for an anatomy teaching specimen, comprising the following steps:
[0050] (1) Preparation of citrus fruit peel extract
[0051] Get the pericarp of citrus fruits, wash and dry, place in a crusher and break into a slurry, after that, add 0.7% of its mass of cellulase and 0.2% of its mass of pectinase to the slurry, stir After uniformity, carry out enzymolysis treatment at 40°C for 2.5 hours. During the enzymolysis treatment, perform ultrasonic treatment for 30 sec...
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