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Method for efficiently obtaining hybrid cymbidium asepsis material by utilizing new lateral buds

A technology of hybrid orchids and lateral buds, applied in the field of plant tissue culture, can solve the problems of unsatisfactory adaptability of imported varieties, high production and operation costs of florists and enterprises, and achieve the goal of reducing the chance of infection, easy infection and increased pollution Effect

Active Publication Date: 2018-12-14
HANGZHOU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the hybrid orchid varieties currently circulating in the domestic market are basically imported from abroad, and the number of self-fertile varieties that are commercialized is almost zero. The source of seedlings is controlled by others. unsatisfactory sex

Method used

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  • Method for efficiently obtaining hybrid cymbidium asepsis material by utilizing new lateral buds
  • Method for efficiently obtaining hybrid cymbidium asepsis material by utilizing new lateral buds
  • Method for efficiently obtaining hybrid cymbidium asepsis material by utilizing new lateral buds

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Experimental program
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Embodiment

[0022] 1. Pretreatment. One week before the material collection, the hybrid orchid plants were treated with 800 times carbendazim solution for root irrigation twice with an interval of 3 days, and the explants were guaranteed to be dry when the materials were collected.

[0023] 2. Sampling. Select healthy new shoots from the strong mother plant, remove impurities and dirt, and bring them back to the laboratory.

[0024] 3. Clean the surface. Peel off the old leaves, scrape off the dirt at the base of the root, brush the surface of the explant with a soft brush dipped in detergent, immerse in 75% alcohol for a few seconds after rinsing, immerse in thick soapy water, shake and clean for 1 hour, and rinse with running water for 1 hour Hour.

[0025] 4. Aseptic disinfection. Blot the moisture on the surface of the explants with absorbent paper, transfer them to an ultra-clean workbench for sterilization, and use alcohol, mercury chloride, sodium hypochlorite and other disinfe...

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Abstract

The invention discloses a method for efficiently obtaining a hybrid cymbidium asepsis material by utilizing new lateral buds. The method relates to the field of plant tissue culture. The invention creates a method for improving a survival rate of small lateral buds in hybrid cymbidium buds. According to the combined use of a sterilizing agent, the pollution rate in the sterile disinfection processis reduced, the obtaining rate of the small lateral buds is improved, and a foundation is laid for seeding propagation of specific individual plants in hybrid cymbidium breeding. According to the method disclosed by the invention, the asepsis material is obtained by taking the small lateral buds produced from the hybrid cymbidium buds as explants, so that the pollution rate can be reduced, the survival rate can be improved, and the method has significances on seeding propagation of the specific individual plants of hybrid cymbidium.

Description

technical field [0001] The invention discloses a method for efficiently obtaining hybrid orchid aseptic materials by utilizing new lateral buds, and the method relates to the field of plant tissue culture. Background technique [0002] The hybrid orchid is an excellent hybrid offspring obtained from the interspecific hybridization of Cymbidium and Cymbidium. Compared with the parent, it has obvious advantages in terms of ornamental, adaptability, production and economic benefits. The hybrid orchid not only inherits the elegant posture and rich fragrance of the Chinese orchid, but also has the advantages of bright colors, large flowers, and a large number of flowers of the Cymbidium. The hybrid orchid has stronger adaptability, can oversummer in flat land, is easy to plant and maintain, and has better economic benefits. Therefore, hybrid orchids have developed rapidly in the orchid industry at home and abroad in recent years. However, the hybrid orchid varieties currently c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/005
Inventor 李春楠傅巧娟沈国正赵福康张晓莹
Owner HANGZHOU ACAD OF AGRI SCI
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