Use of genistein in reducing n-glycolylneuraminic acid in deuterostomes
A technology of genistein and neuraminic acid, applied in the field of genistein reducing N-glycolylneuraminic acid in deuterostomes, can solve the problems of no relevant reports, reduction of superoxide production, etc., and achieve food quality and No effect on nutritional value
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[0014] Animal Feeding:
[0015] Select 80 SPF grade male SD rats at the age of 30 days, and after one week of adaptive feeding, they were randomly divided into two groups: solvent control group (blank control group), genistein group (experimental group), 40 in each group; The temperature of the room is controlled at 23±2°C, the humidity is 60%, and the day and night are alternated for 12 hours; free to eat and drink. The blank group and the experimental group were given 5% ethanol solution and 300mg / kg.d genistein solution by oral gavage respectively.
[0016] Sample Collection:
[0017] After continuous gavage for 15 days, 30 days, 45 days, and 60 days, after the rats in each group could not help water or food, samples of hind legs, kidneys, and livers were taken from 10 rats in the blank control group and the experimental group respectively. Store at -80°C until use.
[0018] Specific methods for sample testing:
[0019] High performance liquid chromatography conditions ...
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