Reagent for rapidly detecting paraquat, and preparation method and application thereof

Abstract

The invention relates to a reagent for rapidly detecting paraquat, and a preparation method and application thereof. The reagent for rapidly detecting the paraquat is prepared by mixing two raw materials of sodium hydrosulfite and soluble carbonate. Compared with an existing detection reagent, the reagent is more convenient to use and higher in use safety. According to the method for rapidly detecting the paraquat, the color can be observed at the sample usage amount of 100-300 mg per 0.3 mL, the effect can be more obvious at the sample usage amount of 100-300 mg per 1 mL, and compared with aconventional test method, the sample usage amount is greatly reduced, the practical application value is wider; through the adoption of the detection method, the test can be completed within several seconds, and the test efficiency is higher under a condition of multiple test samples; and the test proves that 100-300 mg per 1 mL of distilled water is added into the reagent, slight vibration is carried out, a cotton swab is used for dipping a small amount of liquid, and then suspicious spots are wiped, so that the paraquat with the content of more than 0.1 microgram can be detected through thecolor change of the cotton swab, and thus the reagent provided by the invention has a wider test range.

Description

technical field

[0001] The invention belongs to the field of pesticide detection, in particular to a reagent for rapid detection of paraquat, its preparation method and application. Background technique

[0002] Paraquat is a fast-killing herbicide. It can be quickly absorbed by the green tissue of plants, causing them to die. At present, there are a large number of reports of paraquat poisoning at home and abroad. The intake of paraquat is closely related to the prognosis, and it is also the basis for comparing the efficacy of different treatment methods.

[0003] The detection methods of paraquat include blood detection and urine detection. Urine detection is more convenient and quicker, and it is more widely used. However, the current urine detection method needs to add multiple reagents step by step, and each reagent needs to wait for a long time. Waiting for a long time to fully dissolve it before adding another reagent, the detection time is longer, and the amount of...

Examples

Embodiment 1

[0030] This embodiment provides a reagent for rapid detection of paraquat, and the raw material components include:

[0031] Analytical pure sodium dithionite 50mg, analytical pure anhydrous sodium carbonate 50mg.

[0032] Further, a method for preparing the reagent for rapid detection of paraquat is provided, the specific steps are: fully mix analytically pure sodium dithionite and analytically pure anhydrous sodium carbonate in 2mL side 1 / 2 area of ​​PVC coated with white background color In a transparent stoppered test tube, the reagent for rapid detection of paraquat is obtained.

[0033] Further, a method for rapid detection of paraquat is provided, comprising the steps of:

[0034] (1) Drop 0.3mL of the urine to be tested into the reagent for rapid detection of paraquat to obtain the detection solution;

[0035] (2) The detection solution described in step (1) was shaken, and the supernatant showed light blue, that is, the paraquat concentration was 1-4 μg / mL.

Embodiment 2

[0037] This embodiment provides a reagent for rapid detection of paraquat, and the raw material components include:

[0038] Analytical pure sodium dithionite 150mg, analytical pure anhydrous potassium carbonate 150mg.

[0039] Further, a method for preparing the reagent for rapid detection of paraquat is provided. The specific steps are: fully mix analytically pure sodium dithionite and analytically pure anhydrous potassium carbonate in 2 mL of PVC coated with a white background color on the side 1 / 2 area In a transparent stoppered test tube, the reagent for rapid detection of paraquat is obtained.

[0040] Further, a method for rapid detection of paraquat is provided, comprising the steps of:

[0041] (1) Drop 1mL of the urine to be tested into the reagent for rapid detection of paraquat to obtain the detection solution;

[0042] (2) The detection solution described in step (1) was shaken, and the supernatant showed green color, that is, the concentration of paraquat was 4...

Embodiment 3

[0044] This embodiment provides a reagent for rapid detection of paraquat, and the raw material components include:

[0045] Analytical pure sodium dithionite 80mg, analytical pure anhydrous sodium carbonate 80mg.

[0046] Further, a method for preparing the reagent for rapid detection of paraquat is provided, the specific steps are: fully mix analytically pure sodium dithionite and analytically pure anhydrous sodium carbonate in 2mL side 1 / 2 area of ​​PVC coated with white background color In a transparent stoppered test tube, the reagent for rapid detection of paraquat is obtained.

[0047] Further, a method for rapid detection of paraquat is provided, comprising the steps of:

[0048] (1) Drop 1mL of the urine to be tested into the reagent for rapid detection of paraquat to obtain the detection solution;

[0049] (2) The detection solution described in step (1) was shaken, and the supernatant showed brown-green color, that is, the concentration of paraquat was 4-8 μg / mL. ...

technical field

[0001] The invention belongs to the field of pesticide detection, in particular to a reagent for rapid detection of paraquat, its preparation method and application. Background technique

[0002] Paraquat is a fast-killing herbicide. It can be quickly absorbed by the green tissue of plants, causing them to die. At present, there are a large number of reports of paraquat poisoning at home and abroad. The intake of paraquat is closely related to the prognosis, and it is also the basis for comparing the efficacy of different treatment methods.

[0003] The detection methods of paraquat include blood detection and urine detection. Urine detection is more convenient and quicker, and it is more widely used. However, the current urine detection method needs to add multiple reagents step by step, and each reagent needs to wait for a long time. Waiting for a long time to fully dissolve it before adding another reagent, the detection time is longer, and the amount of...

Examples

Embodiment 1

[0030] This embodiment provides a reagent for rapid detection of paraquat, and the raw material components include:

[0031] Analytical pure sodium dithionite 50mg, analytical pure anhydrous sodium carbonate 50mg.

[0032] Further, a method for preparing the reagent for rapid detection of paraquat is provided, the specific steps are: fully mix analytically pure sodium dithionite and analytically pure anhydrous sodium carbonate in 2mL side 1 / 2 area of ​​PVC coated with white background color In a transparent stoppered test tube, the reagent for rapid detection of paraquat is obtained.

[0033] Further, a method for rapid detection of paraquat is provided, comprising the steps of:

[0034] (1) Drop 0.3mL of the urine to be tested into the reagent for rapid detection of paraquat to obtain the detection solution;

[0035] (2) The detection solution described in step (1) was shaken, and the supernatant showed light blue, that is, the paraquat concentration was 1-4 μg / mL.

Embodiment 2

[0037] This embodiment provides a reagent for rapid detection of paraquat, and the raw material components include:

[0038] Analytical pure sodium dithionite 150mg, analytical pure anhydrous potassium carbonate 150mg.

[0039] Further, a method for preparing the reagent for rapid detection of paraquat is provided. The specific steps are: fully mix analytically pure sodium dithionite and analytically pure anhydrous potassium carbonate in 2 mL of PVC coated with a white background color on the side 1 / 2 area In a transparent stoppered test tube, the reagent for rapid detection of paraquat is obtained.

[0040] Further, a method for rapid detection of paraquat is provided, comprising the steps of:

[0041] (1) Drop 1mL of the urine to be tested into the reagent for rapid detection of paraquat to obtain the detection solution;

[0042] (2) The detection solution described in step (1) was shaken, and the supernatant showed green color, that is, the concentration of paraquat was 4...

Embodiment 3

[0044] This embodiment provides a reagent for rapid detection of paraquat, and the raw material components include:

[0045] Analytical pure sodium dithionite 80mg, analytical pure anhydrous sodium carbonate 80mg.

[0046] Further, a method for preparing the reagent for rapid detection of paraquat is provided, the specific steps are: fully mix analytically pure sodium dithionite and analytically pure anhydrous sodium carbonate in 2mL side 1 / 2 area of ​​PVC coated with white background color In a transparent stoppered test tube, the reagent for rapid detection of paraquat is obtained.

[0047] Further, a method for rapid detection of paraquat is provided, comprising the steps of:

[0048] (1) Drop 1mL of the urine to be tested into the reagent for rapid detection of paraquat to obtain the detection solution;

[0049] (2) The detection solution described in step (1) was shaken, and the supernatant showed brown-green color, that is, the concentration of paraquat was 4-8 μg / mL. ...