The invention discloses Fe /
nitrogen co-
doped carbon microspheres as well as a preparation method and application thereof in
uric acid detection, and belongs to the technical field of
analytical chemistry. The preparation method comprises the following steps: dissolving a
carbon source in water, stirring, carrying out
hydrothermal reaction, and washing and
drying an obtained
solid product to obtain carbon microspheres; the preparation method comprises the following steps: putting the carbon microspheres,
ferric salt and a
nitrogen source into water, stirring and
drying, heating, cooling, washing and
drying to obtain the Fe /
nitrogen co-
doped carbon microspheres. The linear response range of the prepared carbon microspheres to
uric acid reaches 14-454 M. In an actual
urine test, the adding standard
recovery rate is stabilized at 95.5-102.2%, the
relative standard deviation is lower than 2.5%, high-sensitivity and high-selectivity detection of
uric acid can be realized, and a new thought is provided for developing a low-cost and high-performance
enzyme-free
biosensor. The uric acid detection method does not need biological
enzyme participation, has the advantages of low cost, good stability, simplicity and convenience in operation and the like, and provides a new technical scheme for clinical uric acid detection.