Human amniotic fluid mesenchymal stem cell transport fluid

A technology for transporting fluid and stem cells, applied in the field of stem cells, can solve the problems of reducing the number of living cells and being unsuitable for long-distance transportation, and achieve the effect of improving the viability rate

Active Publication Date: 2018-12-21
辽宁美全生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of viable cells of the three types of cells (human amniotic fluid, umbilical cord, and placenta-derived mesenchymal stem cells) decreased significantly after being placed in the cell transport solution at room temperature for 72 hours, which is not suitable for long-distance transport

Method used

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Experimental program
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Embodiment Construction

[0014] 1. Experimental materials

[0015] Amniotic fluid from healthy pregnant women in the second trimester was selected, signed informed consent, and agreed to be used in scientific research experiments.

[0016] The special medium for human amniotic fluid mesenchymal stem cells was purchased from BI Company; fetal bovine serum was purchased from Thermo Company of the United States; DMEM medium was purchased from Gibco Company of the United States; The culture medium was purchased from Shanghai Raas Blood Products Co., Ltd.; the stem cell osteogenic differentiation medium was purchased from PromoCell; the neural differentiation medium: 20 μg / L epidermal growth factor and 20 μg / L were added to the DMEM / F12 medium containing 2% B27 Formulated with Basic Fibroblast Growth Factor of L.

[0017] 2. Experimental method

[0018] 1. Isolation and culture of human amniotic fluid mesenchymal stem cells (hAFSCs)

[0019] Dilute the amniotic fluid with PBS containing EDTA at a ratio ...

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Abstract

The invention relates to human amniotic fluid mesenchymal stem cell transport fluid. On the basis of the prior art, 5 microM radix pseudostellariae cyclic peptide A is added, so that the weakness of the prior art that the cell motility rate after the transportation fluid is suspended for 72 hours is apparently reduced can be effectively overcome. The formula of the transport fluid is as follows: the human amniotic fluid mesenchymal stem cell transport fluid is prepared by uniformly mixing 30 percent of 0.2 percent trehalose solution (PBS solution of trehalose), 25 percent of red blood cell storage fluid, 5 percent of 0.2 percent heparin calcium solution (PBS solution of heparin calcium) and 40 percent of human blood albumin injection at normal temperature, and the human amniotic fluid mesenchymal stem cell transport fluid comprises 5 microM radix pseudostellariae cyclic peptide A. The transport fluid not only can improve the motility rate of the human amniotic fluid mesencymal stem cells after being transported for 72 hours, but also does not influence the multi-directional differentiation capability.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a transport solution for human amniotic fluid mesenchymal stem cells. Background technique [0002] Stem cells are a class of cells with self-renewal and multilineage differentiation capabilities. It is because of its regenerative potential and plasticity that it is widely used in regenerative medicine and stem cell research. Stem cells offer hope for many diseases that currently lack effective treatments, including stroke, Huntington's disease, Alzheimer's disease and Parkinson's disease. [0003] Mesenchymal stem cells are multipotent stromal cells isolated from adult and fetal tissues, defined as adherent fibroblast-like cells. Mesenchymal stem cells have intrinsic homing ability, can migrate to damaged tissues, and actively participate in tissue repair. Although mesenchymal stem cells are pluripotent, their self-renewal ability gradually decreases leading to stagnation of cell di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 卢珊珊郑桂纯
Owner 辽宁美全生物科技有限公司
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