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Method for analyzing influences of arbuscular mycorrhizal fungi on plant moisture utilization efficiency

A technology of arbuscular mycorrhizal fungi and plants, applied in the field of botany, can solve unclear problems and achieve the effect of improving plant water use efficiency

Active Publication Date: 2018-12-21
GUIZHOU SERICULTURE RES INST GUIZHOU PEPPER RES INST
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Problems solved by technology

However, whether arbuscular mycorrhizal fungi increase plant WUE by promoting plant growth, regulating root-to-shoot ratio, leaf photosynthetic rate or transpiration rate, or promoting nutrient uptake is still unclear.

Method used

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  • Method for analyzing influences of arbuscular mycorrhizal fungi on plant moisture utilization efficiency

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Embodiment 1

[0018] In the first step, after the required potting soil is collected, 10KGy of cobalt-60 is used to sterilize the soil, and the sterilized soil is packed into plastic buckets, each bucket is filled with 5.0kg of sterilized soil, and arbuscular bacteria are applied. Root fungal agent 50g.

[0019] The second step is to select two mulberry seedlings with the same growth and move them into the sterilized soil plastic bucket containing the fungicide.

[0020] In the third step, during the inoculation and cultivation of mulberry seedlings, all treatments are supplied with normal water, and 200mL / barrel of sterilized water is regularly poured several times a week, the growth temperature is 25-28°C, and the light time is 8-10h / d. After the mulberry tree was cultured for 40 days to form mycorrhiza, no water was supplied, and it was naturally dry for 7 days; then, parameters such as plant height, ground diameter, dry weight of the aboveground part, dry weight of the root, mycorrhizal...

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Abstract

The invention discloses a method for analyzing influences of arbuscular mycorrhizal fungi on plant moisture utilization efficiency. The method comprises the following steps that after soil sterilization, the certain amount of arbuscular mycorrhizal fungi is exerted; target plants are transplanted or seeded; after the plants normally grow for a period of time, water supplying is stopped for seven days or above, all growth indexes start to be monitored, the growing situation, the root shoot ratio, the nutrient and the moisture utilization efficiency of the target plants are calculated, and dataare preserved into the .sav format through an SPSS according to the certain mode; and a structural equation model is built through AMOS software according to the relation between independent variablesand the relation between the independent variables and dependent variables, and reasons for the influences of arbuscular mycorrhizal fungus inoculation on the plant moisture utilization efficiency are explained according to the operation result of the structural equation model. The reasons for improving the plant moisture utilization efficiency can be explained well through a structural equationmodel analyzing method, and the obvious effects are brought to more effective improving of the plant moisture utilization efficiency by further taking manual adjusting and controlling measures duringvegetation recovery of arid regions.

Description

technical field [0001] The invention relates to the field of botany, in particular to a method for analyzing the influence of arbuscular mycorrhizal fungi on plant water use efficiency. Background technique [0002] Water is the main factor limiting plant survival and ecological adaptability. Reasonable and effective use of limited water resources is the prerequisite for long-term stable survival of plants, adaptation to harsh environments, and vegetation restoration and reconstruction. Plant water use efficiency (WUE) can reflect the water use strategy of plants, and is an important physiological and ecological parameter to evaluate the suitability of plants to adversity and measure the drought tolerance of plants. Improving WUE is an urgent problem to be solved in plant production. An important strategic measure for the sustainable development of regional plants. [0003] Arbuscular mycorrhizal fungi can help plants effectively use soil water and nutrients, promote their ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 杨万荣邢丹毛明明罗恒韩世玉张芳
Owner GUIZHOU SERICULTURE RES INST GUIZHOU PEPPER RES INST
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