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Method, measuring instrument and system for detecting urine glucose

A measuring instrument and glucose technology, which is applied in the field of urine glucose detection, can solve the problems of low precision, complex substances, interference, etc., and achieve the effect of high precision and simple operation

Active Publication Date: 2020-10-09
上海荒岛科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, urine test paper is mostly used for clinical urine sugar detection. Urine test paper relies on the glucose in the urine to react with the enzyme on the test paper and change the color of the dye reagent. The change of color reflects the concentration of glucose, but its accuracy is low, and it is a semi-quantitative test.
The electrochemical method is used to test glucose, which has high precision and simple equipment. Blood glucose test strips are very mature in the market, but it is rare to use electrochemical test strips to test urine sugar in the market. The main reason is the substances in urine. It is complicated and seriously interferes with urine sugar testing, such as ascorbic acid, a large number of electrolyte ions and metal ions in urine with high urine specific gravity, which will cause relatively large interference to the electrochemical enzyme sensor for urine sugar testing, resulting in inaccurate test results

Method used

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  • Method, measuring instrument and system for detecting urine glucose
  • Method, measuring instrument and system for detecting urine glucose
  • Method, measuring instrument and system for detecting urine glucose

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Experimental program
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Embodiment 1

[0097] Urine glucose is detected by a system for detecting urine glucose. The system used in this embodiment specifically includes an enzyme-free electrochemical sensor, an enzyme electrochemical sensor and a measuring instrument. The difference between an enzyme-free electrochemical sensor and an enzyme electrochemical sensor is only that there is no enzyme The reagent layer in the electrochemical sensor is an enzyme-free reagent layer, the reagent layer in the enzyme electrochemical sensor is an enzyme reagent layer, and the enzyme-free electrochemical sensor and the enzyme electrochemical sensor can be unified into an electrochemical sensor; figure 1 In the figure, 1 is the substrate, 2 is the working electrode, 3 is the counter electrode, 4 is the trigger electrode, 5 is the insulating film, 6 is the reagent layer, 7 is the hydrophilic film, 8 is the double-sided adhesive layer, 9 is the sample guide groove, and 10 is the gas outlet;

[0098] The surface of the substrate i...

Embodiment 2

[0116] The method that detects glucose in urine is identical with embodiment 1, and difference is: the reagent in the non-enzyme sensor reagent layer and the reagent in the enzyme sensor reagent layer are as shown in table 3 and table 4; Obtain thus: calculation formula is I=(- 0.02852*i+0.67899)*Cglu+(0.85354*i-0.18966);

[0117] Table 3 Composition table of reagents in reagent layer of enzyme-free sensor

[0118] substance mass ratio Potassium ferricyanide 5.4％ Trehalose 1.8％ Hydrophilic silica 1.8％ Tween-20 0.9％ Phosphate buffer solution 45.05％ water 45.05％

[0119] Table 4 Composition of reagents in the enzyme sensor reagent layer

[0120] substance mass ratio glucose oxidase 1.8％ Potassium ferricyanide 5.3％ Trehalose 1.8％ Hydrophilic silica 1.8％ Tween-20 0.9％ Phosphate buffer solution 44.2％ water 44.2％

[0121] Such as Figure 7 As shown, the concentration valu...

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Abstract

The invention provides a method for detecting urine glucose and further provides a measuring instrument and system for detecting the urine glucose. The method comprises the steps that voltage is applied to a non-enzyme electrochemical sensor by the measuring instrument, and a first response current value generated after reacting of the non-enzyme electrochemical sensor with urine is obtained; voltage is applied to an enzyme electrochemical sensor by the measuring instrument, and a second response current value generated after reacting of the enzyme electrochemical sensor with the urine is obtained; and according to the first response current value and the second response current value, the concentration of the urine glucose is calculated. The urine glucose is tested through an electrochemical method, the precision is high, needed equipment is simple, and needed operation is easy.

Description

technical field [0001] The invention relates to the technical field of urine detection, in particular to a method, a measuring instrument and a system for detecting urine glucose. Background technique [0002] Urine routine is a basic routine item for disease detection. Urine specific gravity and urine sugar in urine routine can realize the screening and diagnosis of diseases more intuitively. [0003] Urine sugar test is the easiest way to diagnose diabetes early. Urine sugar will appear after the blood sugar concentration exceeds the renal sugar threshold. The higher the blood sugar concentration, the higher the urine sugar concentration. As a simple and non-invasive detection method, urine glucose detection is a very beneficial method for diabetic patients. [0004] At present, urine test paper is mostly used for clinical urine glucose detection. Urine test paper relies on the glucose in the urine to react with the enzyme on the test paper to change the color of the dye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 王文冲徐晨龙
Owner 上海荒岛科技有限公司
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