Stem cell exosomes, preparation method and application

An exosome and stem cell technology, applied in the field of stem cells, to achieve good skin care effects, improve skin problems, and relieve internal aging effects

Inactive Publication Date: 2018-12-28
深圳市新一仑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of today's skin care products focus on protection, improving or repairing the damage caused

Method used

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  • Stem cell exosomes, preparation method and application
  • Stem cell exosomes, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] User A: Female, 25 years old, with sensitive skin, with red blood streaks and itchy skin all year round. First, use genetic testing to test the following genes:

[0040] Related genes of skin anti-sensitivity: COL5A1, MC1R, KIF3A, OVOL1, SOD1;

[0041] The test results show that the low expression activity of COL5A1 gene of user A leads to thinner skin thickness, while the high expression activity of KIF3A and OVOL1 genes leads to more severe skin allergic reactions than normal people.

[0042] Target gene screening: COL5A1, KIF3A, OVOL1 and SOD1, aiming to increase skin thickness, increase skin light sensitivity and melanin degradation ability, and then improve skin resistance.

[0043] Follow the steps below to prepare stem cell exosomes for user A:

[0044] Isolation and culture of mesenchymal stem cells

[0045] 1) Retrieve the fresh umbilical cord with a sterile bottle (200ml saline + a gentamicin);

[0046] 2) Separate the Wharton’sjelly around the blood vessel and cut it int...

Embodiment 2

[0090] User B: Female, 38 years old, with sagging skin, wrinkles, dryness, and stains. First, use genetic testing to test the following genes.

[0091] Genes related to skin radiation protection and anti-aging ability: XRCC1, RAD51, HBE1, MMP9, SOD1, CHRHR, KL.

[0092] Gene combinations related to anti-wrinkle ability: SOD1, GHRHR, EDAR, FLG, MC1R, MMP9.

[0093] Gene combinations related to the formation of freckles melanin: MC1R, OCA2, BNC2, TYR;

[0094] The test results showed that the expression activity of SOD1, CHRHR, MMP9, and HBE1 genes was low, leading to aging wrinkles, and the lack of TYR gene expression activity resulted in insufficient melanin suppression.

[0095] Target gene screening: select SOD1, CHRHR, MMP9, HBE1 genes as foreign genes to be transferred into mesenchymal stem cells, aiming to improve the skin's anti-aging ability and improve the existing aging state.

[0096] Follow the steps below to prepare stem cell exosomes for user B

[0097] Isolation and culture ...

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Abstract

The invention provides stem cell exosomes, a preparation method and an application. Skin gene detection is performed firstly for skin problems of a user, a target gene for improving the skin problemsof the user is found out according to a gene detection result, the target gene is amplified by in-vitro synthesis and then transferred to a carrier, a target gene carrier is prepared and transfected into mesenchymal stem cells, target gene mesenchymal stem cells are obtained and cultured finally, a cell culture fluid of the cells is collected, the exosomes in the cell culture fluid are extracted,and then the exosomes are sterilized, quantified, subpackaged and stored. Compared with the prior art, the stem cell exosomes, the preparation method and the application have the beneficial effects that problem genes are repaired by starting from gene roots, so that the skin problems can be improved effectively, and internal aging is relieved.

Description

Technical field [0001] The present invention relates to the field of stem cells, in particular to a stem cell exosomes, preparation method and application. Background technique [0002] MSC is currently the most studied stem cell. At present, many clinical trials have confirmed that MSC can treat a variety of diseases and has better effects. At the same time, MSC has stronger safety and can be used in different acid-base environments, hypoxia, high temperature, and repeated freezing. It can still maintain high stability under harsh conditions such as melting, and the functional beauty products made with it can maintain the activity and function of cell regeneration factors to the maximum. Nowadays, there have been many researches on stem cell exosomes skin care products, most of which are Use the activity of its exosomes to improve the skin condition. [0003] However, skin problems are diverse, and user needs are also diverse, such as anti-aging, whitening, anti-allergic, etc., b...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/85A61K8/98A61Q19/00
CPCA61Q19/00A61K8/981A61K8/982A61K8/983C12N5/0663C12N5/0665C12N5/0668C12N15/85C12N2510/00
Inventor 刁波张宜刘莹杨前王刚陈力袁紫林林虹罗青云袁贤玲
Owner 深圳市新一仑生物科技有限公司
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