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Tissue culture and rapid propagation method of wild tinospora capillipes gagnep

A technology of tissue culture rapid propagation and fruit group, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc.

Inactive Publication Date: 2019-01-01
遵义市龙驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Visible, in the prior art, mainly concentrate on adopting the cutting branch of Lavenderia quinquefolium and the seed of Lavenderia quinquefolium to carry out breeding as germplasm resource, also do not see to adopt the young tender stem of Lavenderia quinquefolium as germplasm resource, carry out through tissue culture mode Reports on reproductive treatments

Method used

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  • Tissue culture and rapid propagation method of wild tinospora capillipes gagnep
  • Tissue culture and rapid propagation method of wild tinospora capillipes gagnep

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Cut the young tender stems of Lavenderia japonica into 1-1.5cm sections, inoculate them flat on No. 1 medium for inducing clustered buds and cultivate them for 60 days; cycle, each cycle is 30 days, obtains cluster bud;

[0024] (2) Divide clustered shoots into individual seedlings, transplant to rooted and domesticated seedbeds according to the row-to-plant distance of 5cm×5cm, and domesticate and cultivate for 60 days to obtain the tissue-cultured seedlings of Lavender.

[0025] In the first 15 days of the induction culture of clustered buds, culture was carried out under completely dark conditions, and then culture was carried out under the conditions of light of 1200 Lx and 12 h / d. The described rooting and domestication seedbed is in a simple greenhouse, according to width × length = 1m × 5m, open the compartment, spread a 5cm thick seedling substrate on the compartment surface, and after the clustered bud single plant seedlings are transplanted, build them on ...

Embodiment 2

[0030] On the basis of Example 1, for step (1), after adopting the No. 1 medium for inducing clustered buds to cultivate for 60 days, continue to adopt the subculture of No. 1 medium for inducing clustered buds for two consecutive cycles, each cycle 30 Day, other with embodiment 1.

Embodiment 3

[0032] On the basis of Example 1, for step (1), after adopting No. 2 substratum of clustering bud induction to cultivate for 60 days, continue to adopt the subculture of No. 2 substratum of clustering bud induction for two consecutive cycles, each cycle 30 Day, other with embodiment 1.

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Abstract

The invention relates to the technical field of cultivation of Chinese medicinal materials and in particular relates to a tissue culture and rapid propagation method of wild tinospora capillipes gagnep. According to the tissue culture and rapid propagation method, cluster bud induction culture is carried out on tinospora capillipes gagnep tender stems through two types of cluster bud induction culture media; after cluster buds are divided into single seedlings, the single seedlings are transplanted on a domestication seedling bed, so that the cluster bud induction rate of the tinospora capillipes gagnep obtained by tissue culture propagation reaches 85 percent or more and the survival rate of domestication seedlings reaches 90 percent or more.

Description

technical field [0001] The invention relates to the technical field of cultivation of traditional Chinese medicinal materials, in particular to a method for tissue culture and rapid propagation of wild oleracea. Background technique [0002] Tinospora capillipes Gagnep. is an evergreen twining vine of Tinospora genus. It is mainly produced in Sichuan, Hunan, Guangxi, Hubei, Guizhou and other places. Carbuncle furuncle poison, diarrhea, dysentery, abdominal pain and so on. The roots of Lavender Lavender contain Palnmtine, Colmtfifin, palmatine, jatrorrhizine, African tetrandrine, stephenine, dauricine and magnolialine, which are widely used in folk Treat stomach pain. Modern pharmacological studies have shown that Lavender has obvious anti-inflammatory, analgesic, anti-bacterial, anti-tumor, hypoglycemic, anti-stress and detoxification effects, and is also known as a natural medicine that can replace antibiotics. Due to long-term excavation, the wild resources of jinguolan...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘红美梁大勇石建龙
Owner 遵义市龙驰生物科技有限公司
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