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Application of penthorum Chinense pursh and compounds thereof in preparing medicine for treating atherosclerosis and relieving Alzheimer disease

A technology for atherosclerosis and Alzheimer's disease, which is applied in the field of disease treatment or alleviation by the herb and its compounds, and can solve the problems of unexplored application potential and other problems.

Active Publication Date: 2019-01-04
MACAU UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the yellow grass has been used in folk prescriptions for protecting the liver, its mechanism of action and its application potential in the treatment of other diseases have yet to be explored

Method used

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  • Application of penthorum Chinense pursh and compounds thereof in preparing medicine for treating atherosclerosis and relieving Alzheimer disease
  • Application of penthorum Chinense pursh and compounds thereof in preparing medicine for treating atherosclerosis and relieving Alzheimer disease
  • Application of penthorum Chinense pursh and compounds thereof in preparing medicine for treating atherosclerosis and relieving Alzheimer disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Induction of autophagy by the total extract of C. chinensis

[0067] 1.1 Western blotting to detect the expression of autophagy-related proteins in Hela cells

[0068] Add 15-30 μg / mL drugs to the cultured Hela cells and incubate overnight: the extracts of the flowers of C. chinensis, the extracts of the leaves of C. sylvestris and the stems of C. sylvestris. Rapamycin (Rapa) was used as the autophagy positive control . After drug treatment, RIPA lysate (Cell Signaling Technologies Inc. Beverly, MA, USA) was added to each well at an amount of 70 μl / well to lyse HeLa cells, and Bradford reagent (Bio-Rad, Hercules, CA, USA) was used to measure protein concentration . Add an equal amount of protein to each well of the SDS-PAGE electrophoresis gel, electrophoresis at 300mA for 2 hours, and transfer the protein on the SDS-PAGE gel to the PVDF membrane. The PVDF membrane was blocked with 5% skimmed milk powder for 60 minutes and washed three times with TBST (1X)...

Embodiment 2

[0088] Example 2: Compounds TA, PG, TB, PG1 induce autophagy

[0089] 2.1 Determination of GFP-LC3 green fluorescent particles

[0090] The U87 cell line with GFP-LC3 stably transfected was transfected and established as in Example 1. After drug treatment, GFP-LC3 green fluorescent particles were detected.

[0091] 2.2 Western blotting

[0092] As described in Example 1, 4-8 μM compounds TA, PG, TB, and PG1 were used to treat HUVEC cells, the no-drug group was used as a blank control, and rapamycin (Rapa) was used as a positive control. After drug treatment, the HUVEC cells in each group were lysed by the method described above, SDS-PAGE protein electrophoresis, and the protein was transferred to PVDF membrane. After incubation with antibodies, ECL western blot detection solution (Invitrogen, Paisley, Scotland, UK) was used to expose and develop protein bands.

[0093] Result analysis: The autophagy activity of the above four compounds was analyzed by immunofluorescence tec...

Embodiment 3

[0132] Example 3: TA can reduce the level of oxidative stress in the aorta of the ApoE gene knockout atherosclerosis mouse model (ApoE-KO mice)

[0133] A 2-month-old ApoE gene knockout atherosclerosis mouse (ApoE-KO) model was established, and C57BL / 6J wild-type mice were used as the control group. Each group was given or not given intraperitoneal injection of TA (0.1mg / kg per day) for one month To evaluate the role of TA in the process of early aortic atherosclerosis in ApoE-KO knockout mice.

[0134] 3.1 Measurement of ROS in aortic vessels

[0135] 2',7'-dichlorofluorescin diacetate (2',7'-dichlorofluorescin diacetate, DCFH-DA) was used to measure the intracellular ROS content. Aortic vascular cells of ApoE-KO mice and C57BL / 6J mice were collected by Nylonsieve mesh combined with trypsin method. Add 10 μM DCFH-DA to the above cells, incubate at 37°C for 30 minutes, and then wash three times with serum-free medium. Intracellular esterase hydrolyzes DCFH-DA into DCFH so t...

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Abstract

The invention discloses an application of an extract of penthorum Chinense pursh in preparing medicine for treating atherosclerosis or relieving Alzheimer disease. The extract plays a role through inducing autophagy, and four key compounds in the extract are disclosed. The extract of the penthorum Chinense pursh and the compounds thereof exhibit anti-atherosclerosis oxidative stress damage in experiments in vitro and vivo, have inhibiting effect on formation of beta-amyloid fiber and expression of Tao protein and have potential application for treating atherosclerosis and relieving Alzheimer disease.

Description

technical field [0001] The present invention relates to the application of C. chinensis and its compounds in disease treatment or alleviation, in particular to the application in the preparation of medicines for treating atherosclerosis and alleviating Alzheimer's disease (Alzheimer disease, AD). Background technique [0002] It is a perennial herbaceous plant belonging to the saxifrage family and is a traditional Chinese medicinal material. It is recorded in ancient books that it has the effects of clearing away heat and detoxification, reducing yellowness and dampness, promoting blood circulation and dispelling blood stasis, diuresis and detumescence. figure 1 Shown are the above-ground parts of C. japonica plants, including flowers, leaves and stems (1A), and sun-dried leaves (1B). Modern research through the separation and identification of the effective components of C. chinensis shows that the flavonoids and organic acids contained in it are related to its antioxidant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P9/10A61P25/28
CPCA61K31/7048A61P9/10A61P25/28A61K2300/00
Inventor 黄锦伟罗婉君孙晓磊何延政余录
Owner MACAU UNIV OF SCI & TECH
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