Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of novel iron-oxide nanoparticle and application of nanoparticle to tumor targeted diagnosis and treatment

An iron oxide nanometer and particle technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of short half-life, unsuitable clinical high-throughput testing requirements, radiotoxicity, etc., and achieve strong tissue penetration and high efficiency. Effects of treatment and simultaneous imaging, increased sensitivity and specificity

Active Publication Date: 2019-01-04
ZHENJIANG NO 1 PEOPLES HOSPITAL
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method is sensitive and quantitative, 18F-FDG is not suitable for clinical high-throughput testing requirements due to its short half-life of the isotope.
In addition, radiotracers often have radiotoxicity problems for body organs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of novel iron-oxide nanoparticle and application of nanoparticle to tumor targeted diagnosis and treatment
  • Preparation method of novel iron-oxide nanoparticle and application of nanoparticle to tumor targeted diagnosis and treatment
  • Preparation method of novel iron-oxide nanoparticle and application of nanoparticle to tumor targeted diagnosis and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] A kind of preparation method of novel iron oxide nanoparticle provided by the invention, carry out concrete experiment according to the following steps:

[0032] Step 1: Take 25.3mg of polylactic acid-polyethylene glycol (PLA-PEG), put it in 2mL of dimethylformamide (DMF), and then place the above mixture in 6mL of water for 3h;

[0033] Step 2: Add 550 mg of 1-3-dimethylaminopropyl-3-ethylcarbodiimide hydrochloride (EDC HCl) and 370 mg of N-hydroxysuccinimide (NHS) to the above step After the reaction system of 1, stir for 20min;

[0034] Step 3: After adding 350 mg of glucosamine hydrochloride (DGH) to the reaction system in step 2, stir for 12 hours;

[0035] Step 4: Dialyze and purify the reaction system obtained in step 3 for 24 hours, then filter bacteria through a 0.22 μm filter membrane to obtain PLA-PEG-CO-NHC 6 h 11 o 5 ;

[0036] Step 5: After oleic acid (OA) was conjugated with paclitaxel (PTX), with PLA-PEG-CO-NHC 6 h 11 o 5 OA-PTX-OA-PLA-PEG-CO-NHC...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method of a novel iron-oxide nanoparticle. The preparation method includes the steps: placing polylactic acid-polyethylene glycol and dimethyl formamide into water; adding 1-3-dimethylaminopropyl-3-ethyl carbodiimide hydrochloride and N-hydroxy succinimide into a reaction system; adding glucosamine hydrochloride into the reaction system; performing dialysis, purification and filtration; conjugating oleic acid with taxol, and then mixing the oleic acid with PLA-PEG-CO-NHC6H11O5 by equal molar ratio; adding Fe3O4@OA NPs into ethyl alcohol, adsorbing and separating the system by magnets, and adding tetrahydrofuran into the system to perform ultrasonic oscillation; adding OA-PTX-OA-PLA-PEG-CO-NHC6H11O5 into the system to perform ultrasonic oscillation; dropping the obtained reaction system into the water to perform ultrasonic oscillation, dialysis, purification and filtration. The invention further provides an application of the nanoparticle prepared bythe preparation method to tumor targeted diagnosis and treatment. The novel iron-oxide nanoparticle has dual functions of tumor treatment and tracing, and is high in sensitivity and strong in specificity and penetrability.

Description

technical field [0001] The invention belongs to the field of medical antitumor drugs, and in particular relates to a preparation method of novel iron oxide nanoparticles and its application in targeted diagnosis and treatment of tumors. Background technique [0002] In the past few decades, various anticancer drugs have been widely used in the treatment of tumors. However, antitumor drugs cannot precisely target tumor cells in tumor pathological sites, thus weakening their anticancer effects and causing severe toxicity and side effects. Covalently linking anticancer drugs to ligands that can specifically target and bind tumor cells will increase the therapeutic effect of tumors. At the same time, the real-time in vivo evaluation of tumor treatment effect is of great significance for adjusting treatment plan and tumor prognosis. Existing technologies, such as fluorescence imaging, evaluate chemotherapy drugs, but the safety of the fluorescent reagent itself has problems, an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/08A61K49/18A61K47/54A61K47/69A61K31/337A61K31/7008A61P35/00B82Y5/00B82Y15/00
CPCA61K31/337A61K31/7008A61K49/08A61K49/183A61K47/542A61K47/6935A61K47/6937A61P35/00B82Y5/00B82Y15/00A61K2300/00
Inventor 熊非单秀红顾宁钱炜柳迎昭
Owner ZHENJIANG NO 1 PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com