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Bursa fabricius heptapeptide for promoting vaccine immunoreaction and application thereof

A technology of bursa polypeptide and bursa, which is applied in the field of active heptapeptide of bursa, can solve problems such as economic losses in poultry industry, hidden dangers of public health safety, threats to human health, etc., to promote extensive immune enhancement and simple structure , Improving the effect of vaccine immunity

Active Publication Date: 2019-01-04
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The H9N2 avian influenza virus can not only infect poultry, but also gradually has the ability to infect mammals including humans. a huge threat

Method used

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  • Bursa fabricius heptapeptide for promoting vaccine immunoreaction and application thereof
  • Bursa fabricius heptapeptide for promoting vaccine immunoreaction and application thereof
  • Bursa fabricius heptapeptide for promoting vaccine immunoreaction and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and identification of BP7

[0028] 500 g AA broiler bursa tissue without fascia and adipose tissue was washed once with 0.85% normal saline (pre-chilled to 4-10°C). After draining, place the bursa tissue in a tissue masher, add pre-cooled normal saline, and homogenize at high speed three times for 30 seconds each time. The homogenization process is maintained at 0-10°C. The homogenate was then ultrasonically lysed twice at 4°C, 5 min / time. The lysate was then heated to 80°C for 5 min, then quickly placed on ice and cooled to 10°C. The lysate was then refrigerated and centrifuged for 30 minutes (4000 xg / min). The supernatant was collected and freeze-thawed twice. The supernatant was then centrifuged at high speed at 12000g / min, 4°C, 30min. The supernatant was collected for ultrafiltration, and the ultrafiltrate with a molecular weight below 1000 Da was collected, that is, the crude extract of the bursa tissue. After lyophilization, diluted with ultrapur...

Embodiment 2

[0030] 1. Synthesis of BP7

[0031] A commercial polypeptide synthesis company was entrusted to synthesize the polypeptide according to the GGCDGAA sequence (SEQ ID No. 1), and the purity was required to be ≧97%.

[0032] 2. Vaccines

[0033] AIV inactivated vaccine (purchased from Nanjing Tianbang Biotechnology Co., Ltd.)

[0034] 3. Grouping of experimental animals

[0035] BALB / C mice were randomly divided into five groups, 10 in each group: (I) PBS control group (each immunized with 0.2ml PBS); (II) AIV inactivated vaccine immunization group (each immunized with inactivated vaccine 0.2ml) ); (III-V) inactivated vaccine + BP7 group (BP9 concentrations were 10, 50, 250ug / mL, and the immunization dose was 0.2ml); the corresponding groups of mice were immunized twice by intraperitoneal injection. The immunization interval was two weeks, 200ul / every / every time (Table 1).

[0036] 4. Sample Collection of Immunized Mice

[0037] After the third immunization of mice, the orbi...

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Abstract

The invention relates to separation, authentication and application of a bursa fabricius heptapeptide for promoting vaccine immunoreaction. The immunoactive peptide is a small-molecular polypeptide from bursa fabricius, has the amino acid compoiiton of Gly Gly Cys Asp Gly Ala Ala and is simple in structure and extremeley weak in immunogenicity. The active peptide has a function of promoting the vaccine immunoreaction and has effect of simulating antibody generation and cell immunoreaction, enhancing lymphocyte activity and improving vaccine immunity on mice and chickens. The heptapeptide can be applied to the animal vaccine application research as a vaccine adjuvant or immunopotentiator so as to improve the immune response ability of animal bodies for specific antigens and improve the vaccine immunity, thereby improving the disease infection resistant ability of the animal bodies, and can be applied to the fields of basic immune research, clinical application research and the like.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, in particular to a bursal active heptapeptide capable of promoting vaccine immune response and its application. Background technique [0002] Avian Influenza (AI) is an acute and septic infectious disease caused by influenza A virus, which can infect almost all wild and domestic poultry. Avian influenza virus (AIV) of H9N2 subtype has been widespread in my country and around the world since the 1990s. H9N2 avian influenza virus can not only infect birds, but also has the ability to infect mammals, including humans. a huge threat. Of concern is the 2013 outbreak of H7N9 avian influenza in China, which infects and kills humans. Through genetic analysis of its various fragments, it was found that its internal genes were highly homologous to H9N2, which also confirmed that the virus had undergone recombination. These large-scale reports of avian influenza viruses directly s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61K39/39A61K39/145A61P37/04A61P31/16
CPCA61K38/00A61K39/00A61K39/12C07K7/08C12N2760/16134
Inventor 冯秀丽宗嫚嫚余远楠郑阳曹瑞兵陈溥言
Owner NANJING AGRICULTURAL UNIVERSITY
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