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Fabricius bursa active hexapeptide for promoting immunoreaction of avian influenza and/or Newcastle disease bivalent vaccine and use thereof

A technology of bursa polypeptide and bursa, which is applied in vaccines, multivalent vaccines, veterinary vaccines, etc., can solve the problems of consuming a lot of manpower and material resources, and strengthen the stress response of chicken flocks, so as to improve the antigen presentation response, Improving the ability to resist epidemic infection and improving the effect of vaccine immunity

Active Publication Date: 2019-01-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, immunization with a single vaccine will consume a lot of manpower and material resources, and the increase in the number of immunizations will also strengthen the stress response of the chickens

Method used

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  • Fabricius bursa active hexapeptide for promoting immunoreaction of avian influenza and/or Newcastle disease bivalent vaccine and use thereof
  • Fabricius bursa active hexapeptide for promoting immunoreaction of avian influenza and/or Newcastle disease bivalent vaccine and use thereof
  • Fabricius bursa active hexapeptide for promoting immunoreaction of avian influenza and/or Newcastle disease bivalent vaccine and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Isolation and identification of BP6

[0034] Wash 50 g of AA broiler bursa tissues without fascia and adipose tissue once with 0.85% physiological saline (precooled to 4-10° C.). After draining, place the bursa tissue in a tissue masher, add pre-cooled saline, and homogenize at high speed three times, 30 seconds each time, and keep the homogenization process at 0-10°C. Then, the homogenate was ultrasonically lysed twice at 4°C, 5 min each time. Then the lysate was heated to 80°C, kept warm for 5min, then quickly placed on ice, and cooled to 10°C. The lysate was then refrigerated and centrifuged for 30 minutes (4000×g / min). The supernatant was collected and freeze-thawed twice. Then the supernatant was centrifuged at high speed at 12000g / min, 4°C, 30min. The supernatant was collected for ultrafiltration, and the ultrafiltrate with a molecular weight below 1000 Da was collected, that is, the crude extract of the bursa tissue. After freeze-drying, dilute with ultra...

Embodiment 2

[0036] 1. Synthetic BP6

[0037] A commercial peptide synthesis company was commissioned to synthesize the peptide according to the KGNRVY sequence (SEQ ID No.1), with a purity of 99.936%.

[0038] 2. Vaccines

[0039] Avian influenza and Newcastle disease dual inactivated vaccine (purchased from Nanjing Tianbang Biotechnology Co., Ltd.).

[0040] 3. Grouping of experimental animals and mice

[0041] The BALB / C mice were randomly divided into five groups, 10 in each group: (I) PBS control group (each immunized with 0.2ml PBS); (II) AIV+NDV double inactivated vaccine immunization group (each immunized with inactivated vaccine) live vaccine 0.2ml); (III~V) AIV+NDV double inactivated vaccine+BP6 group (BP6 concentrations were 10, 50, 250 μg / mL); intraperitoneal injection was performed on the corresponding group of mice twice immunity. The immunization interval was two weeks, 0.2ml / each animal / every time (Table 1).

[0042] Table 1 Mixed immunization program of BP6 and AIV+ND...

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Abstract

The invention relates to isolation, identification and application of a Fabricius bursa active hexapeptide for promoting an immunoreaction of avian influenza and / or a Newcastle disease bivalent vaccine. The Fabricius bursa active hexapeptide has an amino acid composition of Lys Gly Asn Arg Val Tyr, has a simple structure and is extremely weak in immunogenicity. The active peptide has the functionof promoting the immune response of the vaccine and can promote the antibody reaction against the AIV antigen and the antibody reaction against the NDV antigen in the mouse in the mouse immunization experiment. The active peptide can promote the cellular immune response, improve lymphocyte viability and an antigen presentation reaction and improve the vaccine immunopotency, can be used as a vaccine adjuvant or immunopotentiator in animal vaccine application research, can improve the immune response of animal organisms to specific antigens, can improve the immune efficacy of vaccines and can improve the ability of animal organisms resisting epidemic disease infection.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a bursa active hexapeptide capable of promoting the immune response of the vaccine avian influenza and / or the dual vaccine with Newcastle disease and its application. Background technique [0002] Avian influenza (Avian influenza, AI) is an infection or disease syndrome caused by avian influenza virus (Avian influenza virus, AIV). After poultry are infected with AIV, some do not show clinical symptoms, and some show a decrease in egg production. And respiratory diseases, and even lead to systemic diseases, the mortality rate can reach 100%. Therefore, avian influenza can be divided into low pathogenic avian influenza (LPAI) and highly pathogenic avian influenza (HPAI) according to the degree of pathogenicity. H9N2 subtype avian influenza virus is one of the most widespread low-pathogenic avian influenza viruses in the world. Its impact on poult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K14/465A61K39/39A61K39/295A61K39/17A61K39/145A61P31/14A61P31/16A61P37/04
CPCA61K39/12A61K39/39A61K2039/552A61K2039/6031A61K2039/70A61P31/14A61P31/16A61P37/04C07K7/06C07K14/465C12N2760/16134C12N2760/18134
Inventor 冯秀丽宗嫚嫚郝珊珊余远楠郑阳陈溥言
Owner NANJING AGRICULTURAL UNIVERSITY
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