Method for researching sand soil solidification effects by carbonic anhydrase mineralization bacteria

A technology of carbonic anhydrase and mineralizing bacteria, applied in the direction of weighing, measuring devices, instruments, etc. by removing certain components

Inactive Publication Date: 2019-01-04
SOUTHWEAT UNIV OF SCI & TECH
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Problems solved by technology

In the prior art, there is no relevant research on whether the content, crystal form and crystal morphol

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  • Method for researching sand soil solidification effects by carbonic anhydrase mineralization bacteria
  • Method for researching sand soil solidification effects by carbonic anhydrase mineralization bacteria
  • Method for researching sand soil solidification effects by carbonic anhydrase mineralization bacteria

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Embodiment Construction

[0058] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0059] 1 Experimental materials

[0060] 1.1 Experimental materials and instruments

[0061] 1.1.1 Bacterial solution

[0062] Beef extract peptone liquid medium was used as the culture medium for the experiment, and the preparation method and culture method of the culture medium were as follows.

[0063] Prepare the bacteria solution to be tested. Prepare beef extract peptone liquid medium, adjust the pH to 7, divide into Erlenmeyer flasks, 100mL per bottle, and put them in a high-temperature sterilizer for sterilization. After the temperature of the culture medium dropped to room temperature, pick a ring of bacteria and inoculate it in the seed liquid culture medium, cultivate it in a shaking incubator at 30°C and 120r / min for 24 hours, and then make a seed liquid. According to the inoculum amount of 1%...

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Abstract

The invention discloses a method for researching sand soil solidification effects by carbonic anhydrase mineralization bacteria. The method includes the steps: firstly, solidification sample preparation; secondly, direct shear experiment; thirdly, calcium carbonate content measurement; fourthly, X-ray diffraction experiment sample preparation; fifthly, electron scanning microscope experiment sample preparation. According to the method, the solidification effects of different grouting modes are explored, sand soil solidification experiments of factors such as calcium source kinds, solidification environment temperature, consolidating fluid concentration and particle diameter are implemented, the solidification effects are compared, and the modes, the concentration and the like with optimalsolidification effects are preferentially selected. The solidification effects of a solidification sample are represented by comparing and analyzing shearing strength and calcium carbonate depositionof the solidification sample under different conditions, a crystal form of calcium carbonate is acquired by an X-ray diffraction instrument, and a microscopic image of the solidification sample is observed by a scanning electron microscope.

Description

technical field [0001] The invention belongs to the technical field of soil reinforcement by microbial grouting, and relates to a research method for the effect of carbonic anhydrase mineralization bacteria on sand stabilization. Background technique [0002] The process of biomineralization and solidification of sandy soil is a process of simultaneous biochemical reactions. The effect of sandy soil solidification is affected by many factors, such as the concentration of bacteria solution, pH, temperature, type of calcium source, concentration of cementing solution, reaction time, Curing conditions, etc. These influencing factors directly affect the strength of the sand specimen after curing (direct shear strength, unconfined compressive strength and triaxial compressive strength, etc.). In the prior art, there is no relevant research on whether the content of calcium carbonate, crystal form and crystal morphology are the main factors affecting the strength of the solidified...

Claims

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Application Information

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IPC IPC(8): G01N3/24G01N5/04G01N23/20
CPCG01N3/24G01N5/04G01N23/20G01N2203/0025G01N2203/0075
Inventor 何鑫李琼芳潘玲于璐嘉王旭辉杨清清宋娜
Owner SOUTHWEAT UNIV OF SCI & TECH
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