Application of dna methylation site of integrin β4 gene in preparation of biomarkers for early diagnosis of asthma and/or copd

A biomarker and early diagnosis technology, applied in the field of biology, can solve problems that cannot be cured, and there are no risk prediction markers

Active Publication Date: 2022-03-18
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In summary, the morbidity and mortality of asthma and COPD have been increasing year by year in recent years, and they still cannot be cured clinically, causing serious family economic burden and social health problems
However, asthma and COPD are complex diseases caused by the interaction of polygenic inheritance and environmental factors, so far there is no effective clinical risk prediction marker

Method used

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  • Application of dna methylation site of integrin β4 gene in preparation of biomarkers for early diagnosis of asthma and/or copd
  • Application of dna methylation site of integrin β4 gene in preparation of biomarkers for early diagnosis of asthma and/or copd

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Experimental program
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Embodiment Construction

[0013] (1) Use MethylTargetTM methylation site evaluation technology to evaluate, clarify the CpG island and other sequence information of integrin β4, provide information for determining candidate research regions, and estimate the number of fragments and systems required.

[0014] Evaluation Criteria

[0015] A. Find the CpG island in the region from 2K upstream of the gene transcription start site to 1K downstream of the first exon (including all splicing modes).

[0016] The parameters for the assessment of methylated CpG islands are:

[0017] Observed / Expected ratio>0.60, Percent C+Percent G>50.00%, Length>200bp.

[0018] B. Fragment Number Evaluation

[0019] a) Conventional (default assessment): each CpG island is expected to design a pair of primers for detection, for longer CpG islands increase (200bp-1K, 1 pair, 1K-2K, 2 pairs, and so on);

[0020] b) Full coverage: calculated according to the length of each CpG island;

[0021] c) Customer-designated interval: d...

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Abstract

The invention discloses the application of the integrin β4 gene DNA methylation site in the preparation of biomarkers for the early diagnosis of asthma and / or COPD.

Description

technical field [0001] The invention belongs to the technical field of biology, and relates to the application of integrin β4 gene DNA methylation site in the preparation of biomarkers for the early diagnosis of asthma and / or COPD, in particular to a biological method for the early diagnosis of asthma and / or COPD Markers and detection methods. Background technique [0002] Bronchial asthma (asthma) and COPD (chronic obstructive pulmonary disease) are common diseases of the respiratory system, but so far the clinical treatment effect of asthma and COPD is unsatisfactory and still cannot be cured, causing great economic and psychological burden to patients , has become a public health issue of global concern. A survey found that the mortality and morbidity of adult asthma and COPD have been increasing in the past 30 years, accounting for 1% of the total global disease burden. It is estimated that asthma, COPD-related lung disease will become the third leading cause of human ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/154
Inventor 刘持秦岭秦晓群袁琳唐莎杜茜子
Owner CENT SOUTH UNIV
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