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Use of human CD147

A CD147, 1. CD147 technology, applied in the field of human CD147 use, can solve problems such as unclear underlying causes

Inactive Publication Date: 2019-01-22
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the underlying causes of most of the

Method used

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  • Use of human CD147

Examples

Experimental program
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Embodiment 1

[0051] In the embodiment of the present invention, the sperm of patients with normal fertility and asthenospermia were respectively used to detect the impact of CD147 deficiency on the normal function of sperm. The CD147 protein in human sperm was localized and quantified by immunofluorescent staining and Western blotting, and the expression and distribution of CD147 protein in sperm from the normal group and the asthenozoospermia group were compared. Correlation analysis of sperm motility.

[0052] 1. Materials and methods

[0053] 1) Reagent

[0054] Normal mouse IgG (Sc-2025) and mouse anti-human CD147 (Sc-20797) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), USA, and discontinuous density gradient sperm separation liquid was purchased from Origio Company (SupraSperm#10970060, Origio Inc.).

[0055] 2) Collection and processing of human sperm samples

[0056] The embodiment of the present invention was approved by the Ethics Committee of the First Affili...

Embodiment 2

[0068] The embodiment of the present invention adopts antibody interference experiment to simulate the expression defect of sperm CD147 protein in vivo, and detect the influence of CD147 protein on sperm motility. At the same time, recombinant human CD147 protein was used to detect whether it had a remedial effect on sperm motility in the asthenozoospermia group.

[0069] The assessment of sperm quality involves other physiological functions besides sperm motility, including sperm capacitation, hyperactivation, acrosome reaction, and sperm-egg binding ability. In the embodiment of the present invention, rCD147 protein was also used to treat the sperm in the sperm of the asthenozoospermia group, and its promoting effect on the function of the sperm of the asthenozoospermia group was examined.

[0070] 1. Materials and methods

[0071] 1) Reagent

[0072] Discontinuous density gradient sperm separation medium (SupraSperm#10970060, Origio Inc.), Quinn's Advantage Fertilization ...

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Abstract

The invention belongs to the technical field of biomedicines, and especially relates to the use of human CD147. In the use of the human CD147, an experiment result shows that the expression of a CD147protein is positively correlated with the sperm motility, and the defect of the CD147 protein can result in low sperm motility; and the expression of the CD147 protein in sperms of an asthenozoospermia group is regulated down, and the application of a recombinant human CD147 protein can significantly improve the acrosome reaction rate of sperms of a patient with asthenospermia and the combining capability of sperms and ovums. The CD147 protein can be used as a biomarker to be applied to prepare a kit for diagnosing the male infertility.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of human CD147. Background technique [0002] Worldwide, the concentration of sperm in men has declined by 57% between 1980 and 2015, and the resulting male infertility has also increased significantly. Similar to the research results of other countries, the sperm quality of Chinese men is gradually declining, and the proportion of male infertility is increasing year by year. According to research, in the past 15 years (2000-2015), the sperm concentration of young men in my country has dropped by 30.8%, and the forward sperm motility has dropped by 38.23%. Asthenozoospermia is a common cause of male infertility, accounting for about 18% of hypofertility or infertility, and is considered to be one of the most important factors affecting the conception of normal sperm and eggs. [0003] Existing studies have shown that many genes are involved in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68A61K45/00A61P15/08
CPCA61K45/00A61P15/08G01N33/6893
Inventor 陈浩陈小章霍建霖史潇赵子建
Owner GUANGDONG UNIV OF TECH
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