Pig sperm diluent and application

A diluent, pig semen technology, applied in the application, preservation of human or animal body, animal husbandry, etc., can solve the problems of poor preservation effect of pig semen at room temperature, poor biocompatibility of antibiotics, short effective storage time, etc., to achieve protection Invariant inactivation, reduced metabolism and exercise levels, long shelf life effects

Inactive Publication Date: 2019-01-25
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This solves the technical problems of poor storage effect of pig semen at room temperature, short ...

Method used

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  • Pig sperm diluent and application
  • Pig sperm diluent and application
  • Pig sperm diluent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Take 35g of glucose, 6g of sodium citrate hydrate, 1.2g of anhydrous sodium bicarbonate, 1.5g of trehalose, 2g of dipotassium edetate, 0.3g of naringin, and 0.40g of berberine, and add them into 950mL of double distilled water and stir well , adjust the pH to 6.8, and then adjust the volume to 1L to obtain a pig semen diluent.

[0041] When in use, add 5 ml of the above diluent to every 1 ml of porcine semen for dilution, and the difference between the temperature of the semen and the dilution temperature during dilution should not exceed 1°C. Store the diluted semen in a 17°C incubator, and mix the semen evenly every 12 hours to prevent sperm precipitation and death.

[0042] figure 1 For the diluent described in embodiment 1 and the pig semen vigor of imported pig semen diluent under different storage times. Imported pig semen diluent 1 was prepared from German Minitu III pig semen dilution powder, and imported pig semen diluent 2 was prepared from Hangzhou Yuefeng ...

Embodiment 2

[0057] Take 20g of glucose, 2g of sodium citrate hydrate, 0.05g of anhydrous sodium bicarbonate, 0.05g of trehalose, 1g of dipotassium edetate, 0.05g of naringin, and 1g of berberine, and add them to 950mL of double-distilled water and stir evenly. Adjust the pH to 7.0, then distill the volume to 1 L with double distilled water to obtain a pig semen diluent.

[0058] When in use, add 4 ml of the above-mentioned porcine semen diluent to every 1 ml of porcine semen for dilution, and the difference between the temperature of the semen and the dilution temperature during dilution should not exceed 1°C. Store the diluted semen in an incubator at 20°C, and mix the semen evenly every 12 hours to prevent sperm precipitation and death.

Embodiment 3

[0060] Take 27g of glucose, 6g of sodium citrate hydrate, 1g of anhydrous sodium bicarbonate, 2g of trehalose, 2g of dipotassium edetate, 0.2g of naringin, and 0.25g of berberine, and add them into 950mL double-distilled water and stir evenly. PH to 7.0, then dilute to 1L with double distilled water.

[0061] When in use, add 5 ml of preservation solution to every 1 ml of porcine semen for dilution, and the difference between the temperature of the semen and the dilution temperature during dilution should not exceed 1°C. Store the diluted semen in a 17°C incubator, and mix the semen evenly every 12 hours to prevent sperm precipitation and death.

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PUM

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Abstract

The invention belongs to the technical field of artificial insemination of pigs, and more specifically relates to a pig sperm diluent and application thereof. The pig sperm diluent is characterized inthat glucose is used as a nutritional agent; sodium citrate hydrate, dipotassium EDTA (Ethylene Diamine Tetraacetate) and anhydrous sodium bicarbonate are used as buffer agents; trehalose is used asa protective agent; berberine is used as an antibacterial agent; naringin is used as an antioxidant, and by utilizing stronger anti-radical action of the antioxidant, sperms are prevented from being subjected to peroxidation damage, and sperm plasma membranes are protected. By adopting the pig sperm diluent disclosed by the invention, the sperm viability is detected to be up to 85 percent after pig sperms are preserved for three days, and the sperm viability still can be enabled to be up to 75 percent after the pig sperms are preserved for seven days.

Description

technical field [0001] The invention belongs to the technical field of pig artificial insemination, and more specifically relates to a pig semen diluent and its application. Background technique [0002] Artificial insemination of pigs is a conventional technology for large-scale pig breeding, which can improve the utilization rate of excellent boars, ensure the high quality of commercial pigs, and increase the economic benefits of farmers. With the wide application of artificial insemination technology, the preservation technology of semen is also developing continuously. The key point of extracorporeal preservation of semen is to slow down the metabolic intensity of sperm, so as to achieve the purpose of prolonging the survival time of sperm in vitro. [0003] Commonly used methods of external preservation of pig semen include semen cryopreservation and semen storage at room temperature. Semen cryopreservation technology mainly uses low temperature to inhibit the metabol...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0231
Inventor 马兆成马海波
Owner HUAZHONG AGRI UNIV
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