Wheat stripe rust-resistant gene QYr.sicau-1B-1 SSR molecular marker and application

A qyr.sicau-1b-1ssr, molecular marker technology, applied in the fields of molecular biology and crop genetics and breeding, can solve the problems of no harvest, inability to perform photosynthesis, yellow leaves of wheat, etc., achieve high linkage degree, improve breeding The effect of work efficiency

Inactive Publication Date: 2019-02-12
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wheat stripe rust is an important disease in my country's wheat production. When it is attacked by stripe rust, yellow necrotic spots will appear on wheat leaves, and yellow uredia will be produced, and these spores will turn black in the later stage; Stripe rust can cause leaves to chlorotic, unable to carry out photosynthesis, thereby reducing yield, and even causing grain failure in severe cases

Method used

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  • Wheat stripe rust-resistant gene QYr.sicau-1B-1 SSR molecular marker and application
  • Wheat stripe rust-resistant gene QYr.sicau-1B-1 SSR molecular marker and application
  • Wheat stripe rust-resistant gene QYr.sicau-1B-1 SSR molecular marker and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Obtainment of Wheat Stripe Rust Resistance Gene QYr.sicau-1B-1 and Its Molecular Marker Xwmc156

[0036] (1) Using '20828' as the female parent and crossing the wheat variety Chuannong 16 as the male parent, the hybrid F1 was obtained. The F1 generation was self-crossed to obtain F2, and 199 individual plants were randomly selected in F2, self-crossed, and harvested separately Single plant, continue selfing in the next year, and repeat this until the F6 generation, and obtain a recombinant inbred line containing 199 lines, constituting a genetic mapping population.

[0037] (2) Identification of stripe rust phenotype in the recombinant inbred line population. When the induced material SY95-71 is fully morbid, the disease resistance response of the test material is recorded in the field, and the response type is classified according to the 1-9 classification standard for each strain in the population. The determination was carried out, and the determination res...

Embodiment 2

[0052] Example 2 Application of Molecular Marker Xwmc156 in Identification of Stripe Rust Resistance QTL QYr.sicau-1B-1

[0053] (1) Use '20828' as the female parent and SY95-71 as the male parent to construct a recombinant inbred line, and randomly select 100 lines from the offspring lines.

[0054] (2) Carry out Xwmc156 marker detection on the obtained 100 strains, the specific method is: extract the DNA of 100 strains at the seedling stage; use it as a substrate, and perform PCR with the specific primer pair of molecular marker Xwmc156 as primers Amplify.

[0055] PCR amplification system: 5μL 10×PCR buffer, 1.5U Ex TaqTM DNA polymerase, 2mmol / lMgCl2, 0.2mmol / L dNTP, 150ng each of upstream and downstream primers, 100ng template DNA, double distilled water to a total of 50μL; Program: Pre-denaturation at 94°C for 5 minutes; denaturation at 94°C for 30s, annealing at 55°C for 30s, and extension at 72°C for 45s, a total of 35 cycles; extension at 72°C for 7 minutes; the obtai...

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Abstract

The invention provides a wheat stripe rust-resistant gene QYr.sicau-1B-1 SSR molecular marker and application. The invention provides a codominant molecular marker Xwmc156 which is located on the wheat 1B chromosome and linkaged with the wheat stripe rust-resistant gene. The molecular marker Xwmc156 is tightly linkaged with the stripe rust-resistant QTL QYr. Sicau-1B-1 on wheat 1B, and can be usedfor positioning the character of the wheat strip rust, accordingly the plants highly sensitive to the stripe rust are eliminated in the breeding process, and the breeding working efficiency is improved. The molecular marker can also be used for detecting the stripe rust-resistant QTL on the wheat 1B chromosome, rapidly screens out the plants with the locus, accordingly can conveniently conduct molecular assistant breeding of high stripe rust-resistant wheat, and has good application prospects.

Description

technical field [0001] The field of molecular biology and crop genetics and breeding of the present invention particularly relates to molecular markers related to wheat stripe rust resistance traits, specifically relates to wheat stripe rust resistance gene QYr. The primer pair and the application of the molecular marker. Background technique [0002] Wheat is an important grain crop in my country, with an annual planting area of ​​more than 20 million hectares, accounting for about 27% of the grain crop area; a total output of more than 100 million tons, accounting for about 22% of the grain crop output. Wheat stripe rust is an important disease in my country's wheat production. When it is attacked by stripe rust, yellow necrotic spots will appear on wheat leaves, and yellow uredia will be produced, and these spores will turn black in the later stage; Stripe rust can cause leaves to chlorotic, unable to carry out photosynthesis, thereby reducing yield, and even cause no har...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 马建
Owner SICHUAN AGRI UNIV
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