Method for inducing siraitia grosvenorii bisexual flowers

A technology of Luo Han Guo and hermaphrodite flowers, which is applied in the field of plant biology, can solve the problems of inability to carry out sex identification at the seedling stage, consume a large amount of labor, and cannot cultivate inbred lines, etc., and achieves the convenience of Luo Han Guo sex molecular marker-assisted breeding and gene editing breeding, reducing The effect of labor intensity

Active Publication Date: 2020-06-16
广西作物遗传改良生物技术重点开放实验室
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Luo Han Guo is usually a dioecious plant, and only the female plant can bear fruit. Artificial pollination is required during the planting process, which requires a lot of labor. It is impossible to cultivate inbred lines during the breeding process. Unable to conduct gender identification, planting male plants wastes a lot of land and money

Method used

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  • Method for inducing siraitia grosvenorii bisexual flowers
  • Method for inducing siraitia grosvenorii bisexual flowers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The budding vine of Luo Han Guo was selected, and the end of the budding vine was moistened with a solution containing an ethylene synthesis inhibitor for 10 seconds. The concentration of the ethylene synthesis inhibitor in the solution was 25 mg / L.

[0022] When the ethylene synthesis inhibitor is (S)-trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid, the preparation method of the solution containing the ethylene synthesis inhibitor is: Weigh (S) )-Trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid is dissolved in dimethyl sulfoxide to form a saturated solution, which is added dropwise to water to form (S)-trans-2- 25mg / L aqueous solution of amino-4-(2-aminoethoxy)-3-butenoic acid.

Embodiment 2

[0024] The budding vine of Luo Han Guo was selected, and the end of the budding vine was moistened with a solution containing an ethylene synthesis inhibitor for 10 seconds. The concentration of the ethylene synthesis inhibitor in the solution was 300 mg / L.

[0025] When the ethylene synthesis inhibitor is (S)-trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid, the preparation method of the solution containing the ethylene synthesis inhibitor is: Weigh (S) )-Trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid is dissolved in dimethyl sulfoxide to form a saturated solution, which is added dropwise to water to form (S)-trans-2- Aqueous solution of 300 mg / L of amino-4-(2-aminoethoxy)-3-butenoic acid.

Embodiment 3

[0027] The budding vine of Luo Han Guo was selected, and the end of the budding vine was moistened with a solution containing ethylene synthesis inhibitor for 50 seconds. The concentration of the ethylene synthesis inhibitor in the solution was 1 mg / L.

[0028] When the ethylene synthesis inhibitor is 1-methylcyclopropene, the preparation method of the solution containing the ethylene synthesis inhibitor is: weigh 1-methylcyclopropene and dissolve it in methanol to form a saturated solution, and add it dropwise to water to form a An aqueous solution of 1 mg / L methylcyclopropene.

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Abstract

The invention discloses a method for inducing siraitia grosvenorii bisexual flowers. The method comprises the following steps that squaring branches and tendrils of siraitia grosvenorii are selected,the ends of the squaring branches and tendrils are wetted with a solution containing an ethylene synthesis inhibitor or an ethylene signal transduction inhibitor for 10 s to 8 h, the concentration ofthe ethylene synthesis inhibitor in the solution is 1 mg/L to 300 mg/L, the concentration of the ethylene signal transduction inhibitor in the solution is 25-500 mg/L; wherein the ethylene synthesis inhibitor is (S)-trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid, 2-aminoisobutyric acid and 1-methylcyclopropene, and the ethylene signal transduction inhibitor is a compound containing silver ions and is any one of silver nitrate and silver thiosulfate. The invention provides a good research basis for the research on the gender regulation and control mechanism and gene mining of siraitia grosvenorii.

Description

Technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for inducing bisexual flowers of Luo Han Guo. Background technique [0002] Luo Han Guo is a precious cucurbitaceous plant unique to our country and is known as a good medicine and fruit. The fruit is rich in glucose, fructose and a variety of vitamins. It has a wide range of uses and is sold well in domestic and foreign markets. It enjoys a high reputation in the international market. It has nourishing lungs and relieving cough, intestinal laxative, anti-oxidation, lowering blood sugar, and Cancer and other effects. Momordica grosvenori is usually a dioecious plant. Only female plants can produce fruit. Artificial pollination is required during the planting process. Artificial pollination requires a lot of labor. Inbred lines cannot be cultivated during the breeding process. The progeny populations are mostly male plants. Unable to con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 莫长明郭文锋李忠
Owner 广西作物遗传改良生物技术重点开放实验室
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