Co-segregated kasp molecular marker of wheat spotted leaf gene lm5 and its application

A technology of molecular markers and co-separation, applied in the field of molecular genetics, can solve the problems of insufficient molecular markers, achieve accurate and efficient detection, improve resistance to stripe rust and powdery mildew, and facilitate and stabilize amplification

Active Publication Date: 2022-04-19
烟台吉恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many closely linked molecular markers that are related to the spotted leaf trait of wheat and can be used in practical molecular breeding.

Method used

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  • Co-segregated kasp molecular marker of wheat spotted leaf gene lm5 and its application
  • Co-segregated kasp molecular marker of wheat spotted leaf gene lm5 and its application
  • Co-segregated kasp molecular marker of wheat spotted leaf gene lm5 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The acquisition of the wheat spotted leaf gene Lm5 and its molecular marker KASP-sicau11

[0038] (1) Using the spotted leaf mutant material MC21 to cross the wheat line 3642 as the male parent, the hybrid F 1 , F 1 F 2 , to obtain F containing 313 plants 2:3 Families, constituting genetic mapping populations.

[0039] (2)F 2:3 Identification of spotted leaf phenotypes in family groups: the leaf color of each line was analyzed and identified at the jointing stage of wheat, the leaves of the entire row of plants were all green and marked as "A", and the leaves of the entire row of plants with all spotted leaves were marked as "B", and the leaves of the entire row of plants with both spots and green are recorded as "H". Each line was judged one by one, and the judgment result was recorded as the phenotypic marker data of the spotted leaf gene Lm5. In this way, the position located on the linkage map is the position of the Lm5 gene.

[0040] (3) BSR-Seq an...

Embodiment 2

[0061] Example 2 Application of Molecular Marker KASP-sicau11 in Selection and Control of Speckled Leaf Gene Lm5

[0062] (1) Using the wheat mutant material MC21 with speckled leaves as the female parent and the common wheat variety CN16 with normal leaves as the male parent to construct F 3 In the generation population, 100 lines were randomly mixed among the offspring lines, and each line contained 10 individual plants.

[0063] (2) Carry out KASP-sicau11 marker detection on the obtained 100 strains, the specific method is: extract the DNA of 100 strains by CTAB method at the seedling stage; Specific primer pairs are primers for fluorescent quantitative PCR amplification, and the primers are:

[0064] Primers on the FAM tag: (the underlined part is the FAM tag sequence) 5'- GAAGGTGACCAAGTTCATGCT TCGCAGTTAAAGCGTCAGC-3' (SEQ ID No. 31);

[0065] Primers on the HEX tag: (the wavy part is the HEX tag sequence) 5'- GAAGGTCGGAGTCAACGGATT TCGCAGTTAAAGCGTCAGT-3' (SEQ ID No. 3...

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Abstract

The invention discloses a KASP molecular marker co-separated with wheat spotted leaf gene Lm5 and an application thereof, belonging to the field of molecular genetics. The KASP molecular marker is KASP‑sicau11, and the polymorphism is C / T; the KASP‑sicau11 molecular marker Co-segregated with the wheat spotted leaf gene Lm5 on the long arm of chromosome 2A; the KASP-sicau11 molecular marker disclosed in the present invention is closely linked with the spotted leaf phenotype resistance gene Lm5, and has high accuracy for molecular marker-assisted selection, so as to quickly screen out Wheat varieties or lines with the Lm5 spotted leaf gene are used for breeding.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a KAS P molecular marker co-separated with the spotted leaf gene Lm5 of wheat and its application. Background technique [0002] Common wheat (Triticum aestivum L.) has a long history of planting, and its planting area accounts for 20%-30% of the cultivated land area in my country, so wheat occupies an important position in my country's crops. The growth and development of food crops are inseparable from the nutrients provided by leaves. As an important organ for photosynthesis in green plants, leaves carry out many important physiological and biochemical reactions. With the continuous development of plant physiology, photosynthesis, photosynthetic pigment synthesis or degradation, photomorphogenesis, plant disease resistance mechanism and programmed cell death are all closely related to plant leaves. Chloroplast is an important organelle for photosynthesis in plants, and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 马建李聪刘航王素容赵聪豪唐华苹牟杨江千涛魏育明郑有良兰秀锦姚琦馥
Owner 烟台吉恩生物科技有限公司
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