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Molecular Marker Primer and Its Application of qgw.nau-4b Main Effect Gene Locus of Wheat Grain Weight

A qgw.nau-4b, molecular marker technology, applied in the field of crop breeding, can solve problems such as thousand-grain weight difference, and achieve the effects of high cost, saving breeding cost, and stable amplification

Inactive Publication Date: 2019-12-27
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the heritability of grain weight is the highest and the impact of the environment is relatively small compared with the other two, in actual production, even for the same variety, there will be large differences in thousand-grain weight in different years and under different environmental conditions

Method used

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  • Molecular Marker Primer and Its Application of qgw.nau-4b Main Effect Gene Locus of Wheat Grain Weight
  • Molecular Marker Primer and Its Application of qgw.nau-4b Main Effect Gene Locus of Wheat Grain Weight
  • Molecular Marker Primer and Its Application of qgw.nau-4b Main Effect Gene Locus of Wheat Grain Weight

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The molecular markers of wheat grain weight main gene QGw.nau‐4B were obtained by the following methods:

[0028] According to the boundary markers Xgwm495 and Xgwm149 of QGw.nau‐4B (Jia et al 2013), the published wheat genome sequence is located in the QGw.nau‐4B segment (http: / / wheat.pw.usda.gov / GG2 / index. shtml) and Wangshuibai BAC clone sequence to screen the polymorphisms of Nanda 2419, Wenmai 6 and Wangshuibai, and finally get two polymorphic markers between the parents, namely WGRC1334 and WGRC1083 Two molecular markers.

[0029] The PCR reaction system is 12.5μl, including 1.25μl of 10×buffer, 25mM MgCl 2 0.75μl, 2.5mMdNTPs 1μl, upstream and downstream primers 0.2μM each, Taq enzyme (5u / μl) 0.1μl, template DNA 10ng, add water to 12.5μl;

[0030] The PCR amplification program was pre-denaturation at 94°C for 3 min, denaturation at 94°C for 30 sec, annealing at 60°C for 1 min, extension at 72°C for 1 min, 35 cycles, and finally 8 min at 72°C; The products were...

Embodiment 2

[0032] Example 2 Molecular marker-assisted transfection of QGw.nau-4B segment increases wheat grain weight

[0033] Using the homozygous near-isogenic genomic DNA of Wenmai No. 6 background as a template, two pairs of molecular marker primers were used to amplify by PCR and detect the amplified products. The amplified fragment of 220bp, and the primer WGRC1083-F and WGRC1083-R of molecular marker WGRC1083 were used to amplify the amplified fragment of 265bp, indicating that the QGw.nau-4B segment was detected in the DNA of the near-isogenic line. Molecular marker primer amplification band patterns see figure 2 and image 3 . Based on the genotypes, identify the grain weight phenotypes of near-isogenic lines and their recipient parents. The results showed that with Wenmai 6 as the control, the 100-kernel weight of the near-isogenic line was significantly increased (Table 1).

[0034] Table 1 The genotype and phenotype of the QGw.nau‐4B near-isogenic line and Wenmai 6 in th...

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Abstract

The invention discloses wheat grain weight major gene locus QGw.nau-4B molecular marker primers and application thereof. In the wheat grain weight major gene locus QGw.nau-4B molecular marker primers, the sequence of the molecular marker primer WGRC1334-F is as shown in SEQ ID NO.1 and the sequence of WGRC1334-R is as shown in SEQ ID NO.2; the sequence of the molecular marker primer WGRC1083-F is as shown in SEQ ID NO.3 and the sequence of the WGRC1083-R is as shown in SEQ ID NO.4. The WGRC1334 and WGRC1083 marked primers are used for detecting a QGW.nau-4B gene, the existence or nonexistence and the existence state of the QGW.nau-4B can be determined, and the grain weight characteristic of the wheat can be predicted, so that plants with the QGw.nau-4B can be quickly screened and the molecular marker primers can be used for improving the wheat variety.

Description

technical field [0001] The invention belongs to the field of crop breeding, and relates to a molecular marker primer of the main gene site QGw.nau-4B for grain weight in wheat and an application thereof. [0002] technical background [0003] Wheat was one of the first food crops domesticated by humans, providing one-fifth of the global population's energy intake. Improving wheat yield has always been the main goal of wheat breeding. Wheat yield is ultimately determined by the number of ears per mu, the number of grains per ear, and the weight of grains. Under high-yield cultivation conditions, when the number of spikes and grains per spike are limited, grain weight is the main factor affecting yield, and increasing grain weight is the key to improving wheat yield potential. Over the past century, there has been a marked increase in kernel weight among newly bred varieties, both in China and elsewhere. Breeding and application of high grain weight varieties is an effective...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 马正强孔忠新程瑞如张利伟
Owner NANJING AGRICULTURAL UNIVERSITY
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