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Kasp-flw-sau6198 Molecular Marker Linked to the Major QTL of Wheat Flag Leaf Width and Its Application

A kasp-flw-sau6198, molecular marker technology, applied in the fields of molecular biology and crop genetics and breeding, can solve the problem of not many molecular markers, and achieve the effects of accurate and efficient detection, convenient and stable amplification, and high accuracy

Active Publication Date: 2022-07-01
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many tightly linked molecular markers that are related to the trait of wheat flag leaf width and can be used in practical molecular breeding.

Method used

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  • Kasp-flw-sau6198 Molecular Marker Linked to the Major QTL of Wheat Flag Leaf Width and Its Application
  • Kasp-flw-sau6198 Molecular Marker Linked to the Major QTL of Wheat Flag Leaf Width and Its Application
  • Kasp-flw-sau6198 Molecular Marker Linked to the Major QTL of Wheat Flag Leaf Width and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Acquisition of the wheat flag leaf width QTL QFLW.sau-AM-4B.4 and its molecular marker KASP-Flw-sau6198

[0043] (1) Using the wheat line 'Ailanmai' as the female parent, and crossing the wheat variety 'LM001' as the male parent, the hybrid F was obtained. 1 , F 1 Generation of individual plants to obtain F by selfing 2 , in F 2 Use the single spike method, all the way to F 8 For generations, a recombinant inbred line containing 121 lines was obtained, which constituted a genetic mapping population.

[0044] (2) Identification of the flag leaf width phenotype of the recombinant inbred line population: The flag leaf width of the recombinant inbred line was analyzed and identified at the maturity stage of wheat. Flag leaf width, and obtain the average value, representing the flag leaf width of the line.

[0045] (3) 55K SNP chip analysis

[0046] a) DNA extraction: DNA was extracted from the parental 'Ailanmai', 'LM001' and the recombinant inbred line popu...

Embodiment 2

[0058] Example 2 Application of molecular marker KASP-Flw-sau6198 in selection and control of flag leaf width QTL QFLW.sau-AM-4B.4

[0059] (1) Recombinant inbred lines were constructed using the tetraploid wheat 'PI 503554' with narrower flag leaves as the male parent and the tetraploid endemic wheat 'Ailanmai' with wider flag leaves as the female parent, and randomly selected from the progeny lines 54 strains.

[0060] (2) Carry out KASP-Flw-sau6198 labeling detection on the obtained 54 strains. The specific method is as follows: extracting the DNA of 54 strains; using it as a template, molecularly marking the specific primer pair of KASP-Flw-sau6198 PCR amplification and fluorescence readings were performed for primers that were:

[0061] Primer on FAM tag: (the underlined part is the FAM tag sequence) 5'- GAAGGTGACCAAGTTCATGCT TCGACAGCCGGACTTCTCGA-3' (SEQ ID No. 1)

[0062] Primer on the HEX tag: (the wavy line part is the HEX tag sequence) 5'-

[0063]

[0064] Un...

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Abstract

The invention discloses a KASP-Flw-sau6198 molecular marker linked with the main effect QTL of wheat flag leaf width and its application, belonging to the fields of molecular biology and crop genetics and breeding. The molecular marker is a SNP molecular marker, and the polymorphism is A / G, which co-localizes with the wheat flag leaf width QTL QFLW.sau-AM-4B.4 on the long arm of the tetraploid wheat chromosome 4B, and is located in the QTL QFLW .sau‑AM‑4B.4 range. After detection and analysis, it is shown that the molecular marker can accurately track the flag leaf width QTLQFLW.sau‑AM‑4B.4 of wheat, and predict the flag leaf width characteristics of wheat, which is conducive to more rapid screening of wheat varieties or lines with wider flag leaves For breeding, greatly speed up the process of wheat breeding.

Description

technical field [0001] The invention relates to the fields of molecular biology and crop genetics and breeding, in particular to the KASP-Flw-sau6198 molecular marker linked with the major QTL of wheat flag leaf width and its application. Background technique [0002] Wheat (Triticum sp.) is one of the most widely grown crops in the world. Facing the growing world population, how to improve wheat yield has become a key issue for breeders. [0003] Flag leaf is the main photosynthetic organ of wheat, which affects the accumulation of dry matter and the formation of yield. Therefore, the size of the flag leaf has a great influence on the final yield of wheat. Flag leaf width is an important agronomic trait, not only one of the important components of wheat plant type, but also the main organ of plant photosynthesis, which plays an important role in yield potential in wheat. During the grain filling stage, half of the organic matter of the seeds is provided by flag leaves, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6851C12N15/11
CPCC12Q1/6895C12Q1/6851C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 马建王健赵聪豪王素容曾照勇刘航唐华苹牟杨邓梅苟璐璐谭翠江千涛魏育明郑有良兰秀锦
Owner SICHUAN AGRI UNIV
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